Journal ArticleDOI
α-Amylase immobilization on the silica nanoparticles for cleaning performance towards starch soils in laundry detergents
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TLDR
In this article, α-amylase was immobilized on silica nanoparticles and the immobilized αamylases was used in formulation of detergent powder for enhancing removal of starch soils.Abstract:
In this study, α-amylase was immobilized on silica nanoparticles and immobilized α-amylase was used in formulation of detergent powder for enhancing removal of starch soils. Detergent products contain very components which may affect the free enzyme activity and stability. Also various factors such as temperature, pH and humidity reduced enzyme activity and cleaning efficiency. Therefore the effect of enzyme immobilization on the removal of starch based soil was investigated on cotton fabrics as the model soil. The effect of temperature and humidity on stability of free and immobilized enzyme was compared. It was found that the immobilized enzyme increased cleaning efficiency toward starch soil removal on cotton fabrics, whereas free enzyme imposed a small effect on the enzymatic activity towards the same soil substrates. In addition, stability immobilized enzyme against temperature and humidity was much more than free enzyme.read more
Citations
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Journal ArticleDOI
Enzyme immobilization: an overview on techniques and support materials.
TL;DR: Future investigations should endeavor at adopting logistic and sensible entrapment techniques along with innovatively modified supports to improve the state of enzyme immobilization and provide new perspectives to the industrial sector.
Journal ArticleDOI
An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
Nur Royhaila Mohamad,Nur Haziqah Che Marzuki,Nor Aziah Buang,Fahrul Huyop,Roswanira Abdul Wahab +4 more
TL;DR: Characterization technologies at the nanoscale level to study enzymes immobilized on surfaces are crucial to obtain valuable qualitative and quantitative information, including morphological visualization of the immobilized enzymes, to assess efficacy of an immobilization technique and development of future enzyme immobilization strategies.
Journal ArticleDOI
Inorganic Materials as Supports for Covalent Enzyme Immobilization: Methods and Mechanisms
Paolo Zucca,Enrico Sanjust +1 more
TL;DR: The most common inorganic supports for covalent immobilization of the enzymes are reviewed, with particular focus on their advantages and disadvantages in terms of enzyme loadings, operational stability, undesired adsorption, and costs.
Journal ArticleDOI
Principles, techniques, and applications of biocatalyst immobilization for industrial application
TL;DR: This review focuses on most commonly used immobilization techniques in industry with many recent applications including using bioreactor systems for industrial production.
Journal ArticleDOI
Enzyme Immobilization: An Overview on Nanoparticles as ImmobilizationMatrix
Razi Ahmad,Meryam Sardar +1 more
TL;DR: This review presents the current scenario and techniques in enzyme immobilization, and the main methods used to combine proteins/enzymes with nanoparticles is given in the study.
References
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Journal ArticleDOI
Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose
TL;DR: In this article, a number of surfactants were screened for their ability to improve enzymatic hydrolysis of steam-pretreated spruce (SPS), and non-ionic surfactant was found to be the most effective.
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Entrapping enzyme in a functionalized nanoporous support.
TL;DR: OPH entrapped in the organically functionalized nanopores showed an exceptional high immobilization efficiency of more than 200% and enhanced stability far exceeding that of the free enzyme in solution.
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Aqueous Sol−Gel Process for Protein Encapsulation
TL;DR: A novel aqueous sol−gel process to encapsulate biological molecules (such as enzymes, antibodies, and cells) that uses neutral pH and room temperature and does not generate alcohol as a byproduct is developed.
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Anisotropic orientation in molecular monolayers by infrared spectroscopy
TL;DR: In this paper, Fourier transform IR measurements of Langmuir-Blodgett monolayers have been made with both grazing incidence reflection and transmission techniques, and the implication of these results for characterizing the orientational anisotropy of chemical groups in thin polymer films is also discussed.
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Enhancement of enzymatic hydrolysis of cellulose by surfactant
TL;DR: Tween 20 plays an important role in the hydrolysis of crystalline cellulose and that Tween 20 disturbs the adsorption of endoglucanase on cellulose, resulting in enhancing the reaction.