Journal ArticleDOI
A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries.
Hai Yu,Harshal A. Chokhawala,Rebekah Karpel,Hui Yu,Bingyuan Wu,Jianbo Zhang,Yingxin Zhang,Qiang Jia,Xi Chen +8 more
TLDR
A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph).Abstract:
A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained protein is (1) an alpha2,3-sialyltransferase (main function), (2) an alpha2,6-sialyltransferase, (3) an alpha2,3-sialidase, and (4) an alpha2,3-trans-sialidase. The recombinant tPm0188Ph is a powerful tool in the synthesis of structurally diverse sialoside libraries due to its relaxed substrate specificity, high solubility, high expression level, and multifunctionality.read more
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Glycosyltransferases: structures, functions, and mechanisms.
TL;DR: The expected two-step double-displacement mechanism is rendered less likely by the lack of conserved architecture in the region where a catalytic nucleophile would be expected, and a mechanism involving a short-lived oxocarbenium ion intermediate now seems the most likely, with the leaving phosphate serving as the base.
Journal ArticleDOI
Advances in the Biology and Chemistry of Sialic Acids
Xi Chen,Ajit Varki +1 more
TL;DR: A large library of sialoside standards and derivatives in amounts sufficient for structure-activity relationship studies are provided and sialoglycan microarrays provide an efficient platform for quick identification of preferred ligands for sialic acid-binding proteins.
Journal ArticleDOI
Pasteurella multocida pathogenesis: 125 years after Pasteur
TL;DR: Pasteurella multocida was first shown to be the causative agent of fowl cholera by Louis Pasteur in 1881 and a number of other virulence factors have been identified by both directed and random mutagenesis, including PMT, putative surface adhesins and iron acquisition proteins.
Journal ArticleDOI
Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: Potential implications for disease
Vered Padler-Karavani,Hai Yu,Hongzhi Cao,Harshal A. Chokhawala,Felix Karp,Nissi Varki,Xi Chen,Ajit Varki +7 more
TL;DR: It is suggested that this ongoing antigen-antibody reaction may generate chronic inflammation, possibly contributing to the high frequency of diet-related carcinomas and other diseases in humans.
Journal ArticleDOI
Enzymatic labeling of proteins: techniques and approaches
TL;DR: This review article summarizes the enzymatic strategies, which enable site-specific functionalization of proteins with a variety of different functional groups, which include formylglycine generating enzyme, sialyl transferases, phosphopantetheinyltransferases, O-GlcNAc post-translational modification, sortagging, transglutaminase, farnesyltransferase, biotin ligase, lipoic acid ligase and N-myristoyltransferase
References
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Eliana Saxon,Carolyn R. Bertozzi +1 more
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Chemical Diversity in the Sialic Acids and Related α-Keto Acids: An Evolutionary Perspective
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TL;DR: The present state of knowledge in sialobiology, with an emphasis on my personal experience in this research area, is outlined including a discussion of necessary future work in this fascinating field of cell biology.
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