Journal ArticleDOI
A new technique to expand human mesenchymal stem cells using basement membrane extracellular matrix.
Takehiro Matsubara,Shinichi Tsutsumi,Haiou Pan,Hisatada Hiraoka,Ryo Oda,Masahiro Nishimura,Hiroshi Kawaguchi,Kouzou Nakamura,Yukio Kato +8 more
TLDR
The growth rate and the proliferative life span of the stem cells markedly increased using tissue culture dishes coated with a basement membrane-like extracellular matrix, which was produced by PYS-2 cells or primary endothelial cells.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 2004-01-16. It has received 114 citations till now. The article focuses on the topics: Clinical uses of mesenchymal stem cells & Stem cell.read more
Citations
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Aging of mesenchymal stem cells
TL;DR: While the literature might often appear to conflict, many apparent discrepancies are attributable to inconsistent methods of extracting and isolating MSC which in fact contains various subsets of adult stem cells, varying not only in their differentiation potential but also in their vulnerability to senescence--ranging from quasi-somatic lifespan to perennial vigour.
Journal ArticleDOI
Dynamic Three-Dimensional Culture Methods Enhance Mesenchymal Stem Cell Properties and Increase Therapeutic Potential
TL;DR: This work demonstrates that these methods for in vitro MSC culture are a viable alternative to monolayer techniques and may prove beneficial for retaining MSC properties in vitro and suggests a novel therapeutic application for dynamic 3D MSCs.
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Role of Adult Mesenchymal Stem Cells in Bone Tissue Engineering Applications: Current Status and Future Prospects
TL;DR: This review highlights advances and progress in bone reconstruction techniques for both the repair of site-specific bone defects and the attenuation of musculoskeletal disease symptoms associated with osteoporosis and osteogenesis imperfecta.
Journal ArticleDOI
Mesenchymal stem cell aging.
TL;DR: Current knowledge about MSC aging (in vitro or in vivo), and respective difficulties for tissue engineering and stem cell therapy are reviewed.
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Three-dimensional nanofibrillar surfaces promote self-renewal in mouse embryonic stem cells.
TL;DR: Support is provided for the view that the three‐dimensionality of the culture surface may function as a cue for the activation of Rac and PI3K signaling pathways, resulting in stem cell proliferation and self‐renewal.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum
TL;DR: A rapid method has been devised which requires only 5 c.mm.
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Mesenchymal stem cells: Biology and potential clinical uses
TL;DR: Preclinical and early clinical safety studies are paving the way for further applications of mesenchymal stem cells in the field of transplantation with respect to hematopoietic support, immunoregulation, and graft facilitation.
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A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.
TL;DR: A rapid spectrophotometric procedure is described for the estimation of sulfated glycosaminoglycans in cartilage cultures that is substantially free from interference, is sensitive to less than 1 microgram (4 micrograms/ml) of chondroitin sulfate, and provides a simple alternative to the traditional methods for gly cosaminoglycan determinations.
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Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model
TL;DR: The data suggest a possible model of predetermined bone marrow stromal cells differentiation where the tripotent cells can be considered as early mesenchymal progenitor that display a sequential loss of lineage potentials, generating osteochondrogenic progenitors which, in turn, give rise to osteogenic precursors.