Journal ArticleDOI
A Porous Silicon-Based Optical Interferometric Biosensor
Victor Lin,Kianoush Motesharei,Kianoush Motesharei,Keiki-Pua S. Dancil,Keiki-Pua S. Dancil,Michael J. Sailor,Michael J. Sailor,M. Reza Ghadiri,M. Reza Ghadiri +8 more
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TLDR
A biosensor has been developed based on induced wavelength shifts in the Fabry-Perot fringes in the visible-light reflection spectrum of appropriately derivatized thin films of porous silicon semiconductors based on Binding of molecules induced changes in the refractive index of the porous silicon.Abstract:
A biosensor has been developed based on induced wavelength shifts in the Fabry-Perot fringes in the visible-light reflection spectrum of appropriately derivatized thin films of porous silicon semiconductors. Binding of molecules induced changes in the refractive index of the porous silicon. The validity and sensitivity of the system are demonstrated for small organic molecules (biotin and digoxigenin), 16-nucleotide DNA oligomers, and proteins (streptavidin and antibodies) at pico- and femtomolar analyte concentrations. The sensor is also highly effective for detecting single and multilayered molecular assemblies.read more
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Sensitive optical biosensors for unlabeled targets: a review.
TL;DR: This article reviews the recent progress in optical biosensors that use the label-free detection protocol, in which biomolecules are unlabeled or unmodified, and are detected in their natural forms, and focuses on the optical biosENSors that utilize the refractive index change as the sensing transduction signal.
Journal ArticleDOI
Novel Mesoporous Materials with a Uniform Distribution of Organic Groups and Inorganic Oxide in Their Frameworks
TL;DR: In this article, the synthesis of highly ordered organic-inorganic hybrid mesoporous materials is described. But the synthesis procedure to polymerize the organosilane monomer containing two trialkoxysilyl groups in the presence of surfactant can be applied to synthesize a variety of high-order mesopore materials.
Journal ArticleDOI
Porous silicon: a quantum sponge structure for silicon based optoelectronics
TL;DR: The photoluminescence properties of porous silicon have attracted considerable research interest since their discovery in 1990 as discussed by the authors, which is due to excitonic recombination quantum confined in Si nanocrystals which remain after the partial electrochemical dissolution of silicon.
Journal ArticleDOI
Metal-assisted chemical etching in HF/H2O2 produces porous silicon
Xiuling Li,Paul W. Bohn +1 more
TL;DR: In this paper, a simple and effective method is presented for producing light-emitting porous silicon (PSi) using a thin layer of Au, Pt, or Au/Pd is deposited on the (100) Si surface prior to immersion in a solution of HF and H2O2 depending on the type of metal deposited and Si doping type and doping level.
References
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Quantitative monitoring of gene expression patterns with a complementary DNA microarray.
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Accessing Genetic Information with High-Density DNA Arrays
Mark S. Chee,Robert Yang,Earl Hubbell,Anthony Berno,Xiaohua C. Huang,David Stern,Jim Winkler,David J. Lockhart,Macdonald S. Morris,Stephen P. A. Fodor +9 more
TL;DR: The simultaneous analysis of the entire human mitochondrial genome is described here and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability.
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Control of protein-ligand recognition using a stimuli-responsive polymer
Patrick S. Stayton,Tsuyoshi Shimoboji,Tsuyoshi Shimoboji,Cynthia J. Long,Ashutosh Chilkoti,Guohua Chen,J. M. Harris,Allan S. Hoffman +7 more
TL;DR: It is shown that conjugating a temperaturesensitive polymer to a genetically engineered site on a protein allows the protein's ligand binding affinity to be controlled, and environmentally triggered control of binding may find many applications in biotechnology and biomedicine.
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A fiber-optic DNA biosensor microarray for the analysis of gene expression.
TL;DR: A fiber-optic biosensor array is described that enables fast and sensitive detection of multiple DNA sequences simultaneously, with the potential for quantitative hybridization analysis.
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Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids
Edwin M. Southern,Stephen C. Case-Green,John Kenneth Elder,Matthew P. Johnson,Kalim U. Mir,L Wang,Jennifer C. Williams +6 more
TL;DR: The array is used in a hybridisation reaction to a labelled target sequence, and shows the hybridisation behaviour of every oligonucleotide in the target sequence with its complement in the array.