Journal ArticleDOI
A Protein Antibiotic in the Phage Qβ Virion: Diversity in Lysis Targets
Reads0
Chats0
TLDR
The two small phages characterized for their lysis strategy, Qβ and the small DNA phage φX174, effect host lysis by targeting different enzymes in the multistep, universally conserved pathway of cell wall biosynthesis.Abstract:
A2, a capsid protein of RNA phage Qβ, is also responsible for host lysis. A2 blocked synthesis of murein precursors in vivo by inhibiting MurA, the catalyst of the committed step of murein biosynthesis. An A2-resistance mutation mapped to an exposed surface near the substrate-binding cleft of MurA. Moreover, purified Qβ virions inhibited wild-type MurA, but not the mutant MurA, in vitro. Thus, the two small phages characterized for their lysis strategy, Qβ and the small DNA phage φX174, effect host lysis by targeting different enzymes in the multistep, universally conserved pathway of cell wall biosynthesis.read more
Citations
More filters
Journal ArticleDOI
Bacteriophage T4 Genome
TL;DR: T4 functional genomics will aid in the interpretation of these newly sequenced T4-related genomes and in broadening the understanding of the complex evolution and ecology of phages—the most abundant and among the most ancient biological entities on Earth.
Journal ArticleDOI
Rapid Killing of Streptococcus pneumoniae with a Bacteriophage Cell Wall Hydrolase
TL;DR: It is shown here that seconds after contact, a purified pneumococcal bacteriophage lytic enzyme (Pal) is able to kill 15 common serotypes of pneumococci, including highly penicillin-resistant strains.
Journal ArticleDOI
Bacteriophage endolysins--current state of research and applications.
TL;DR: Owing to their specificity and high activity, endolysins have been employed for various in vitro and in vivo aims, in food science, in microbial diagnostics, and for treatment of experimental infections.
Journal ArticleDOI
Phage genomics: small is beautiful.
Harald Brüssow,Roger W. Hendrix +1 more
TL;DR: Comparative Genomics addresses--for the first time at whole genome resolution--a set of fundamental biological questions related to populations: What is the structure of the global phage population?
Journal ArticleDOI
Bacteriophage therapy : a revitalized therapy against bacterial infectious diseases
Shigenobu Matsuzaki,Mohammad Rashel,Jumpei Uchiyama,Shingo Sakurai,Takako Ujihara,Masayuki Kuroda,Masahiko Ikeuchi,Toshikazu Tani,Mikiya Fujieda,Hiroshi Wakiguchi,Shosuke Imai +10 more
TL;DR: Phage therapy will compensate for unavoidable complications of chemotherapy such as the appearance of multidrug resistance or substituted microbism, and phage-coded inhibitors of peptidoglycan synthesis (protein antibiotics), search methods for novel antibacterial agents using phage genome informatics, and vaccines utilizing phages or their products are being developed.
References
More filters
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Independent and Tight Regulation of Transcriptional Units in Escherichia Coli Via the LacR/O, the TetR/O and AraC/I1-I2 Regulatory Elements
Rolf Lutz,Hermann Bujard +1 more
TL;DR: Controlling the expression of the genes encoding luciferase, the low abundance E.coli protein DnaJ and restriction endonuclease Cfr9I not only demonstrates that high levels of expression can be achieved but also suggests that under conditions of optimal repression only around one mRNA every 3rd generation is produced.
Journal ArticleDOI
A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.
Mitchell Singer,Tania A. Baker,G Schnitzler,S M Deischel,M Goel,W Dove,K J Jaacks,Alan D. Grossman,James W. Erickson,Carol A. Gross +9 more
TL;DR: A collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome, designed to be used in a rapid two-step mapping system in E. coli.
Journal ArticleDOI
Holins: the protein clocks of bacteriophage infections.
TL;DR: Two proteins, an endolysin and a holin, are essential for host lysis by bacteriophage, and constitute one of the most diverse functional groups, with >100 known or putative holin sequences, which form >30 ortholog groups.
Journal ArticleDOI
Phages will out: strategies of host cell lysis
TL;DR: Most phages accomplish host lysis using a muralytic enzyme, or endolysin, and a holin, which permeabilizes the membrane at a programmed time and thus controls the length of the vegetative cycle.