A ratiometric pH reporter for imaging protein-dye conjugates in living cells.
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TLDR
A molecule that transfers energy through bonds from a donor to an acceptor was prepared with a pH sensitive donor function (fluorescein) and this probe was used to image a conjugate of the probe with bovine serum albumin that was imported into endosomes or in the cytosol using the noncovalently bound carrier.Abstract:
A molecule that transfers energy through bonds from a donor to an acceptor was prepared with a pH sensitive donor function (fluorescein). At pH values above 6.5, minimal energy transfer occurred, and the probe emitted green fluorescence (ca. 520 nm) when excited at the donor (488 nm). Below pH 6.0 however, energy transfer is efficient; hence excitation at the donor causes emission at the acceptor part (600 nm). This probe was used to image a conjugate of the probe with bovine serum albumin that was imported into endosomes or in the cytosol using the noncovalently bound carrier, Pep-1, at 37 and 4 °C, respectively. The more acidic environment of the endosomes was conspicuous from the red fluorescence of the probe.read more
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References
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Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ.
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Acidification of the endocytic and exocytic pathways
TL;DR: The Exocytic Pathway and the CharacTERISTICS of PROTON ATPases are reviewed.
Journal Article
Cellular pH gradient in tumor versus normal tissue : Potential exploitation for the treatment of cancer
Leo E. Gerweck,Kala Seetharaman +1 more
TL;DR: Electro-measured pH values of human tumors and adjacent normal tissues consistently show that the electrode pH is substantially and consistently lower in the tumor than in normal tissue, and the 31P-magnetic resonance spectroscopy estimated that intracellular pH is essentially identical or slightly more basic in tumor compared to normal tissue.
Journal ArticleDOI
Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.
TL;DR: The pH of the medial/trans-Golgi was measured and demonstrated that the Golgi membrane in intact cells is relatively permeable to H+, and that Cl- serves as a counter-ion for H+ transport and likely helps to maintain electroneutrality.
Journal ArticleDOI
A peptide carrier for the delivery of biologically active proteins into mammalian cells.
TL;DR: A new strategy for protein delivery based on a short amphipathic peptide carrier, Pep-1, which is able to efficiently deliver a variety of peptides and proteins into several cell lines in a fully biologically active form, without the need for prior chemical covalent coupling or denaturation steps.
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