A reduced protein diet induces stearoyl-CoA desaturase protein expression in pig muscle but not in subcutaneous adipose tissue: relationship with intramuscular lipid formation.
Reads0
Chats0
TLDR
The results suggest that an increase in intramuscular but not subcutaneous adipose tissue fatty acids under the influence of a RPD is related to tissue-specific activation of SCD expression, and it is suggested that the SCD isoform spectra in pig sub cutaneous adiposes tissue and muscle might be different.Abstract:
A reduced protein diet (RPD) is known to increase the level of intramuscular lipid in pig meat with a smaller effect on the amount of subcutaneous adipose tissue. This might be due to tissue-specific activation of the expression of lipogenic enzymes by the RPD. The present study investigated the effect of a RPD, containing palm kernel oil, soyabean oil or palm oil on the activity and expression of one of the major lipogenic enzymes, stearoyl-CoA desaturase (SCD) and on the level of total lipids and the fatty acid composition of muscle and subcutaneous adipose tissue in pigs. The RPD significantly increased SCD protein expression and activity in muscle but not in subcutaneous adipose tissue. The level of MUFA and total fatty acids in muscle was also elevated when the RPD was fed, with only small changes in subcutaneous adipose tissue. A positive significant correlation between SCD protein expression and total fatty acids in muscle was found. The results suggest that an increase in intramuscular but not subcutaneous adipose tissue fatty acids under the influence of a RPD is related to tissue-specific activation of SCD expression. It is suggested that the SCD isoform spectra in pig subcutaneous adipose tissue and muscle might be different.read more
Citations
More filters
Journal ArticleDOI
Fat deposition, fatty acid composition and meat quality: A review
J. D. Wood,MB Enser,AV Fisher,G.R. Nute,P.R. Sheard,R. I. Richardson,SI Hughes,F. M. Whittington +7 more
TL;DR: It is shown that a major factor is the total amount of fat and that phospholipid, where 18:2n-6 is located, declines as a proportion of muscle lipid and the proportion of neutral lipid, with its higher content of saturated and monounsaturated fatty acids, increases.
Journal ArticleDOI
Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers.
Jean-François Hocquette,Florence Gondret,Elisabeth Baéza,Françoise Médale,Catherine Jurie,David W. Pethick +5 more
TL;DR: The knowledge on fat accumulation in muscles is now being underpinned by the exhaustive nature of genomics, and efforts have concentrated on discovering DNA markers that change the distribution of fat in the body at the expense of carcass fatness.
Journal ArticleDOI
Advances in low-protein diets for swine.
TL;DR: This paper reviews recent advances in the study of LP diets for swine, provides some insights into future research directions, and addresses concerns of increased fatness when pigs are fed low-protein (LP) diets.
Journal ArticleDOI
Intermuscular and intramuscular adipose tissues: Bad vs. good adipose tissues.
TL;DR: It seems that in the pig and humans, INTMF and VAT share a similar pattern of distribution and a similar association of components dictating insulin sensitivity, while in pigs, the relationship between leanness and higher proportions of IN TMF fat in pigs was not observed in human studies and was not corroborated by other pig studies.
Journal ArticleDOI
Δ9 desaturase protein expression and fatty acid composition of longissimus dorsi muscle in lambs fed green herbage or concentrate with or without added tannins
Valentina Vasta,Alessandro Priolo,M. Scerra,Katharine G. Hallett,J. D. Wood,Olena Doran,Olena Doran +6 more
TL;DR: It was suggested that in ruminants, in contrast to monogastric animals, Δ(9)d expression does not play the key role in intramuscular fatty acids formation and tannins action on the enzyme expression needs to be elucidated.
References
More filters
Journal ArticleDOI
Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials.
R P Mensink,M B Katan +1 more
TL;DR: All fatty acids elevated HDL cholesterol when substituted for carbohydrates, but the effect diminished with increasing unsaturation of the fatty acids, and that for monounsaturates was not.
Journal ArticleDOI
Role for Stearoyl-CoA Desaturase-1 in Leptin-Mediated Weight Loss
Paul Cohen,Makoto Miyazaki,Nicholas D. Socci,Aaron Hagge-Greenberg,Wolfgang Liedtke,Alexander A. Soukas,Ratnendra Sharma,Lisa C. Hudgins,James M. Ntambi,Jeffrey M. Friedman +9 more
TL;DR: Ob/ob mice with mutations in SCD-1 were significantly less obese than ob/ob controls and had markedly increased energy expenditure, suggesting that down-regulation of SCD1 is an important component of leptin's metabolic actions.
Journal ArticleDOI
Regulation of stearoyl-coa desaturase by polyunsaturated fatty acids and cholesterol
TL;DR: The goal of this review is to provide an overview of the genetic regulation of the stearoyl-CoA desaturase in response to dietary polyunsaturated fatty acids and cholesterol.
Journal ArticleDOI
Dietary lipids and blood cholesterol: quantitative meta-analysis of metabolic ward studies.
TL;DR: In this article, a meta-analysis of metabolic ward studies of solid food diets in healthy volunteers was conducted to determine the quantitative importance of dietary fatty acids and dietary cholesterol to blood concentrations of total, low density lipoprotein, and high density LDL cholesterol.
Journal ArticleDOI
Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid.
TL;DR: Inhibition and analog studies suggest that the methylene chain of stearyl-CoA assumes an eclipsed ("gauche") conformation at carbon atoms 9,10 in the enzyme-substrate complex of desaturase, and isotope rate effects obtained with deuterated st earyl- CoA derivatives indicate that hydrogen removal is the rate-limiting step of desaturation.