Open AccessBook
A short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria
TLDR
Experiments in Molecular Genetics as mentioned in this paper is a spiral bound manual for those doing genetic or recombinant DNA work with E. coli or similar organisms with step-by-step protocols and clear diagrams that demonstrate major concepAbstract:
A sequel to Experiments in Molecular Genetics (Cold Spring Harbor Lab. Press, 1972) for those doing genetic or recombinant DNA work with E. coli or similar organisms. The spiral-bound manual includes 34 detailed experiments with step-by-step protocols and clear diagrams that demonstrate major concepread more
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Journal ArticleDOI
Stochastic Gene Expression in a Single Cell
TL;DR: This work constructed strains of Escherichia coli that enable detection of noise and discrimination between the two mechanisms by which it is generated and reveals how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation.
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Real-Time Kinetics of Gene Activity in Individual Bacteria
TL;DR: This work directly demonstrates transcriptional bursting in Escherichia coli, similar to that indirectly inferred for eukaryotes, and extends protein-based approaches by counting the integer-valued number of transcript with single-molecule resolution.
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Antibiotic tolerance facilitates the evolution of resistance.
TL;DR: In vitro evolution experiments found that in all cases, tolerance preceded resistance, and a mathematical population-genetics model showed how tolerance boosts the chances for resistance mutations to spread in the population.
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Quorum sensing in Escherichia coli and Salmonella typhimurium
TL;DR: In this article, the authors show that E. coli and S. typhimurium produce a small soluble heat labile organic molecule that is involved in intercellular communication, but the molecule is not produced when the strains are grown in Luria-Bertani medium in the absence of glucose.
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Issues in cDNA microarray analysis: quality filtering, channel normalization, models of variations and assessment of gene effects
TL;DR: A quality index, computed from duplicate spots on the same slide, is used to filter out outlying spots, poor quality genes and problematical slides and a rank invariant method is suggested to select non-differentially expressed genes and to construct normalization curves in comparative experiments.