Acetogenic bacteria utilize light-driven electrons as an energy source for autotrophic growth.
Sangrak Jin,Yale Jeon,Min Soo Jeon,Jongoh Shin,Yoseb Song,Seulgi Kang,Jiyun Bae,Suhyung Cho,Jung-Kul Lee,Dong Rip Kim,Byung-Kwan Cho +10 more
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In this paper, a nanoparticle-microbe hybrid system was developed in which chemically synthesized cadmium sulfide nanoparticles (CdS-NPs) were displayed on the cell surface of the industrial acetogen Clostridium autoethanogenum.Abstract:
Acetogenic bacteria use cellular redox energy to convert CO2 to acetate using the Wood-Ljungdahl (WL) pathway. Such redox energy can be derived from electrons generated from H2 as well as from inorganic materials, such as photoresponsive semiconductors. We have developed a nanoparticle-microbe hybrid system in which chemically synthesized cadmium sulfide nanoparticles (CdS-NPs) are displayed on the cell surface of the industrial acetogen Clostridium autoethanogenum The hybrid system converts CO2 into acetate without the need for additional energy sources, such as H2, and uses only light-induced electrons from CdS-NPs. To elucidate the underlying mechanism by which C. autoethanogenum uses electrons generated from external energy sources to reduce CO2, we performed transcriptional analysis. Our results indicate that genes encoding the metal ion or flavin-binding proteins were highly up-regulated under CdS-driven autotrophic conditions along with the activation of genes associated with the WL pathway and energy conservation system. Furthermore, the addition of these cofactors increased the CO2 fixation rate under light-exposure conditions. Our results demonstrate the potential to improve the efficiency of artificial photosynthesis systems based on acetogenic bacteria integrated with photoresponsive nanoparticles.read more
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Construction of CdS-Tetrahymena thermophila hybrid system by efficient cadmium adsorption for dye removal under light irradiation.
TL;DR: In this article , a Tetrahymena thermophila, a single-celled ciliate protozoa, was used to enrich and remove the heavy metal Cd2+ from water.
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Novel approaches to energize microbial biocatalysts
TL;DR: S semiconductor nanoparticles that absorb light and transfer electrons (photoelectrons) behaving as artificial photosynthetic systems (biohybrid systems) are discussed, and some major limitations/challenges and future prospects of SNs as microbial energization systems are discussed.
Journal ArticleDOI
Metabolic changes of the acetogen Clostridium sp. AWRP through adaptation to acetate challenge
TL;DR: In this paper , the phenotypic changes that occurred in the acetogenic bacterium Clostridium sp. showed that the adapted strain showed a higher cell density than AWRP even without exogenous acetate supplementation.
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Light-driven ammonium oxidation to dinitrogen gas by self-photosensitized biohybrid anammox systems
Mei Zuo Guo,Sen Qiao +1 more
TL;DR: In this article , an anammox-cadmium sulfide nanoparticles (CdS NPs) biohybrid system was constructed for nitrogen removal from water/wastewater.
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