Adding new chemistries to the central dogma of molecular biology
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TLDR
A case study of the successful addition of new chemistries to the fundamental processes of the central dogma of molecular biology, exemplified by the expansion of the genetic code is presented.About:
This article is published in Chem.The article was published on 2021-11-11 and is currently open access. It has received 8 citations till now. The article focuses on the topics: Central dogma of molecular biology.read more
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Chemical Conjugation to Less Targeted Proteinogenic Amino Acids
TL;DR: In recent years a number of methods have emerged for chemical bioconjugation to other amino acids and in this review, the progress in this area is presented.
Posted ContentDOI
An efficient opal-suppressor tryptophanyl pair creates new routes for simultaneously incorporating up to three distinct noncanonical amino acids into proteins in mammalian cells
TL;DR: The bacteria-derived tryptophanyl (EcTrp) pair is an excellent TGA-suppressor in mammalian cells and it is shown that this pair does not cross-react with any of the three previously established aaRS/tRNA pairs.
Journal ArticleDOI
Site-Specific Introduction of Bioorthogonal Handles to Nanopores by Genetic Code Expansion.
Jing Yang,Kefan Wang,Shanyu Zhang,Xiaocui Zheng,Tongxiao Cui,Xiande Yang,Yao Liu,Di Lu,Yuqi Wang,Xiao Tian,Panke Zhang,Shuo Hao Huang,Ran Xie +12 more
TL;DR: In this paper , the authors reported the genetic code expansion (GCE) strategy to introduce unnatural amino acid (UAA) to an octameric Mycobacterium smegmatis porin A (MspA) nanopore.
Posted ContentDOI
Development, Characterization, and Structural Analysis of a Genetically Encoded Red Fluorescent Peroxynitrite Biosensor
TL;DR: In this paper , a boronic acid-containing noncanonical amino acid (ncAA), p-boronophenylalanine (pBoF), was introduced to amino acid residues spatially close to the chromophore of an enhanced circularly permuted red fluorescent protein (ecpApple).
References
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Journal ArticleDOI
Click Chemistry: Diverse Chemical Function from a Few Good Reactions.
TL;DR: In this paper, a set of powerful, highly reliable, and selective reactions for the rapid synthesis of useful new compounds and combinatorial libraries through heteroatom links (C-X-C), an approach called click chemistry is defined, enabled, and constrained by a handful of nearly perfect "springloaded" reactions.
Journal ArticleDOI
Central Dogma of Molecular Biology
TL;DR: The central dogma of molecular biology deals with the detailed residue-by-residue transfer of sequential information and states that such information cannot be transferred from protein to either protein or nucleic acid.
Journal ArticleDOI
Adding New Chemistries to the Genetic Code
Chang C. Liu,Peter G. Schultz +1 more
TL;DR: The development of new orthogonal aminoacyl-tRNA synthetase/tRNA pairs has led to the addition of approximately 70 unnatural amino acids to the genetic codes of Escherichia coli, yeast, and mammalian cells, which provide new opportunities to generate proteins with enhanced or novel properties and probes of protein structure and function.
Journal ArticleDOI
Expanding the Genetic Code of Escherichia coli
TL;DR: A unique transfer RNA/aminoacyl-tRNA synthetase pair has been generated that expands the number of genetically encoded amino acids in Escherichia coli and should provide a general method for increasing the genetic repertoire of living cells to include a variety of amino acids with novel structural, chemical, and physical properties not found in the common 20 amino acids.
Journal ArticleDOI
Cu-free click cycloaddition reactions in chemical biology
TL;DR: This tutorial review will summarize the history of this emerging field, as well as recent progress in the development and application of bioorthogonal copper-free click cycloaddition reactions.