Journal ArticleDOI
Advanced molecular immunoassay system for immunobiotic lactic acid bacteria using a transfectant of Toll-like receptor 2
Masanori Tohno,Takeshi Shimosato,Yasushi Kawai,Hisashi Aso,Shuji Ikegami,Naoki Taketomo,Tadao Saito,Haruki Kitazawa +7 more
TLDR
Results suggest that sTLR2-expressing HEK293 cells may be useful in certain molecular immunoassay systems for producing new physiologically functional foods with intestinal immunomodulatory abilities, such as the maintenance of Th1/Th2 polarization.Abstract:
Toll-like receptor 2 (TLR2) is a receptor for a variety of microbial components, and it also mediates activation signals in the cell relating to the innate immune system. In order to evaluate the precise molecular immunoregulation by various strains of lactic acid bacteria (LAB) via TLR2, the swine TLR2 (sTLR2)-expressing transfectant was constructed using human embryonic kidney (HEK) 293 cells. It is demonstrated that intact immunobiotic LAB can induce immune responses through TLR2, and that different nuclear factor-κB (NF-κB) activities of various strains can be accurately detected by sTLR2-expressing HEK293 cells. Furthermore, cellular activation of NF-κB via TLR2 is reflected in enhanced binding and uptake of LAB. The sTLR2-expressing HEK293 cells were also useful for characterizing the expression pattern of type I helper T (Th1) and type II helper T (Th2) cytokines by the stimulation of immunobiotic LAB. These results suggest that sTLR2-expressing HEK293 cells may be useful in certain molecular immunoassay systems for producing new physiologically functional foods with intestinal immunomodulatory abilities, such as the maintenance of Th1/Th2 polarization.read more
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The cell wall of lactic acid bacteria: Surface constituents and macromolecular conformations
TL;DR: The present work demonstrates that the impressive variation in surface properties displayed by even a limited number of genetically-related bacterial strains can be understood in terms of established colloidal concepts, provided that sufficiently detailed structural, chemical, and conformational information on the surface constituents is available.
Journal ArticleDOI
Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway
Tomoyuki Shimazu,Julio Villena,Masanori Tohno,Hitomi Fujie,Shoichi Hosoya,Takeshi Shimosato,Hisashi Aso,Yoshihito Suda,Yasushi Kawai,Tadao Saito,Seiya Makino,Shuji Ikegami,Hiroyuki Itoh,Haruki Kitazawa +13 more
TL;DR: The results indicated that PIE cells can be used to study the mechanisms involved in the protective activity of immunobiotics against intestinal inflammatory damage and may provide useful information for the development of new immunologically functional feeds that help to prevent inflammatory intestinal disorders, including weaning-associated intestinal inflammation.
Journal ArticleDOI
Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line.
Masayuki Moue,Masanori Tohno,Tomoyuki Shimazu,Taketomo Kido,Hisashi Aso,Tadao Saito,Haruki Kitazawa +6 more
TL;DR: PIE induces inflammatory responses by up-regulating Th1 cytokines and chemokines in response to LPS or ETEC, suggesting that PIE is a useful cell line for studying inflammatory responses via TLR4/MD-2 in intestinal epithelial cells.
Journal ArticleDOI
Immunomodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets
Kan Sato,Kazuaki Takahashi,Masanori Tohno,Masanori Tohno,Y. Miura,T. Kamada,Shuji Ikegami,Haruki Kitazawa +7 more
TL;DR: The results show that immunobiotics, particularly L. gasseri TL2919, might be useful as immunomodulators to stimulate the gut-associated immune system in neonatal chicks, and thereby protect them from disease without decreasing growth performance as a possible substitution of antibiotics.
Journal ArticleDOI
Lactic acid bacteria in the prevention of pneumococcal respiratory infection: future opportunities and challenges.
TL;DR: Research from the last decade demonstrates that LAB represent a promising resource for the development of prevention strategies against respiratory infections that could be effective tools for medical application.
References
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