Advances in Gene Delivery Systems.
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TLDR
The rationale for the design of viral, nonviral and physical methods for gene delivery, as well as advantages and disadvantages of each of the most commonly used gene delivery methods, are explained and future perspectives are provided.Abstract:
The transfer of genes into cells, both in vitro and in vivo, is critical for studying gene function and conducting gene therapy. Methods that utilize viral and nonviral vectors, as well as physical approaches, have been explored. Viral vector-mediated gene transfer employs replication-deficient viruses such as retro-virus, adenovirus, adeno-associated virus and herpes simplex virus. A major advantage of viral vectors is their high gene delivery efficiency. The nonviral vectors developed so far include cationic liposomes, cationic polymers, synthetic peptides and naturally occurring compounds. These nonviral vectors appear to be highly effective in gene delivery to cultured cells in vitro but are significantly less effective in vivo. Physical methods utilize mechanical pressure, electric shock or hydrodynamic force to transiently permeate the cell membrane to transfer DNA into target cells. They are simpler than viral- and nonviral-based systems and highly effective for localized gene delivery. The past decade has seen significant efforts to establish the most desirable method for safe, effective and target-specific gene delivery, and good progress has been made. The objectives of this review are to (i) explain the rationale for the design of viral, nonviral and physical methods for gene delivery; (ii) provide a summary on recent advances in gene transfer technology; (iii) discuss advantages and disadvantages of each of the most commonly used gene delivery methods; and (iv) provide future perspectives.read more
Citations
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Smart micro/nanoparticles in stimulus-responsive drug/gene delivery systems
Mahdi Karimi,Amir Ghasemi,Parham Sahandi Zangabad,Reza Rahighi,S. Masoud Moosavi Basri,S. Masoud Moosavi Basri,Hamed Mirshekari,Mandana Amiri,Z. Shafaei Pishabad,A. Aslani,Mahnaz Bozorgomid,Deepanjan Ghosh,Ali Beyzavi,A. Vaseghi,Amir Reza Aref,L. Haghani,Sajad Bahrami,Michael R. Hamblin,Michael R. Hamblin +18 more
TL;DR: This review highlights the recent advances of smart MNPs categorized according to their activation stimulus (physical, chemical, or biological) and looks forward to future pharmaceutical applications.
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Triazine dendrimers as drug delivery systems: From synthesis to therapy
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Exosomes Derived from miR-126-modified MSCs Promote Angiogenesis and Neurogenesis and Attenuate Apoptosis after Spinal Cord Injury in Rats.
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Glycolysis inhibition as a cancer treatment and its role in an anti-tumour immune response.
Kheshwant S. Gill,Philana Fernandes,Tracey R. O’Donovan,Sharon L. McKenna,Kishore K. Doddakula,Derek G. Power,Declan M. Soden,Patrick F. Forde +7 more
TL;DR: This review examines the interplay between the glycolysis modulation and the immune response as an anti-cancer therapy and looks at the glyCOlysis pathway as a target for cancer treatments.
References
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TL;DR: The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy.
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