Airway epithelial regeneration requires autophagy and glucose metabolism
Kuan Li,Minmin Li,Wenli Li,Hongzhi Yu,Xin Sun,Qiuyang Zhang,Yu Li,Xue Li,Yue Li,E. Dale Abel,Qi Wu,Huaiyong Chen,Huaiyong Chen +12 more
TLDR
It is suggested that autophagy and glucose metabolism are essential for the maintenance of airway epithelium at steady state and during allergic inflammation.Abstract:
Efficient repair of injured epithelium by airway progenitor cells could prevent acute inflammation from progressing into chronic phase in lung. Here, we used small molecules, genetic loss-of-function, organoid cultures, and in vivo lung-injury models to show that autophagy is essential for maintaining the pool of airway stem-like vClub cells by promoting their proliferation during ovalbumin-induced acute inflammation. Mechanistically, impaired autophagy disrupted glucose uptake in vClub progenitor cells, and either reduced accessibility to glucose or partial inhibition of glycolysis promoted the proliferative capacity of vClub progenitor cells and their daughter Club cells. However, glucose deprivation or glycolysis blockade abrogated the proliferative capacity of airway vClub cells and Club cells but promoted ciliated and goblet cell differentiation. Deficiency of glucose transporter-1 suppressed the proliferative capacity of airway progenitor cells after ovalbumin challenge. These findings suggested that autophagy and glucose metabolism are essential for the maintenance of airway epithelium at steady state and during allergic inflammation.read more
Citations
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Role of Autophagy in Lung Inflammation.
TL;DR: The role of autophagy in non-immune cells, myeloid cells, and lymphoid cells for their implications into lung inflammation and asthma is discussed and the role viral pathogenesis, immunometabolism, and asthma are discussed.
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Organoid models in lung regeneration and cancer
TL;DR: The current data on lung epithelial regeneration by regional endogenous stem/progenitor cells, the development of organoid technology, and its applications in lung cancer research are summarized.
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Lung organoids, useful tools for investigating epithelial repair after lung injury.
Jing Kong,Jing Kong,Shiyuan Wen,Wenjing Cao,Peng Yue,Xin Xu,Yu Zhang,Lisha Luo,Taigui Chen,Lianbao Li,Feng Wang,Jian Tao,Guozhong Zhou,Suyi Luo,Aihua Liu,Fukai Bao +15 more
TL;DR: In this article, the authors review the recent methods of cultivating lung organoids, applications of lung organoid in epithelial repair after injury, and understand the mechanisms of epithelial repairing investigated using lung organizers.
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SARS-CoV-2 Infection and Lung Regeneration
TL;DR: It is proposed that, in addition to symptomatic treatments being developed and applied in the clinic, targeting lung regeneration is also essential to restore lung homeostasis in COVID-19 patients.
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The role of autophagy in the metabolism and differentiation of stem cells.
Maryam Adelipour,Leena Regi Saleth,Saeid Ghavami,Keshav Narayan Alagarsamy,Sanjiv Dhingra,Abdolamir Allameh +5 more
TL;DR: The role of autophagy in stem cell proliferation, differentiation, self-renewal, and senescence is well documented in this article , and the potential of targeting autophagous stem cells for future therapies is discussed.
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