An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.
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TLDR
Oligonucleotide probes with deoxyinosine residues at ambiguous points seem to be useful as hybridization probes for cloning genes for proteins containing amino acids with degenerate codons.About:
This article is published in Journal of Biological Chemistry.The article was published on 1985-03-10 and is currently open access. It has received 507 citations till now. The article focuses on the topics: Amino acid & Oligonucleotide.read more
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Characterization and chromosomal localization of a cDNA encoding brain amyloid of Alzheimer's disease
TL;DR: Four clones were isolated from an adult human brain complementary DNA library with an oligonucleotide probe corresponding to the first 20 amino acids of the beta peptide of brain amyloid from Alzheimer's disease, and the 3.5-kilobase messenger RNA was detected in mammalian brains and human thymus.
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Phylogenetic Stains: Ribosomal RNA-Based Probes for the Identification of Single Cells
TL;DR: Quantitative microfluorimetry shows that the amount of an rRNA-specific probe that binds to Escherichia coli varies with the ribosome content and therefore reflects growth rate.
Journal ArticleDOI
Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells
Lawrence M. Souza,Thomas C. Boone,Janice Gabrilove,Por H. Lai,Krisztina M. Zsebo,Murdock Douglas C,Vicki R. Chazin,Joan Bruszewski,Hsieng Sen Lu,Kenneth Chen,Jean M. Barendt,Erich Platzer,Malcolm A.S. Moore,Roland Mertelsmann,Karl Welte +14 more
TL;DR: Experiments were conducted to isolate and characterize the gene and gene product of a human hematopoietic colony-stimulating factor with pluripotent biological activities, which has the ability to induce differentiation of a murine myelomonocytic leukemia cell line WEHI-3B(D+) and cells from patients with newly diagnosed acute nonlymphocytic cancer.
Journal ArticleDOI
Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.
TL;DR: Cloning of a cDNA encoding the inducible nitric oxide synthase from a murine macrophage cell line, RAW264.7, exposed to interferon-gamma and lipopolysaccharide, identifies shared binding sites for the cofactors NADPH and flavins in the C-terminal half of both proteins and an additional conserved region near the N terminus that may recognize L-arginine and/or contribute to the active site.
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Insulin-like growth factor II receptor as a multifunctional binding protein.
David O. Morgan,David O. Morgan,Jeffrey C. Edman,David Standring,Victor A. Fried,Michele C. Smith,Richard A. Roth,William J. Rutter +7 more
TL;DR: The primary structure of human insulin-like growth factor II receptor, predicted from the complementary DNA sequence, reveals a transmembrane receptor molecule with a large extracellular domain made up of fifteen repeat sequences and a small region homologous to the collagen-binding domain of fibronectin.
References
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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
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Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
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A complementation analysis of the restriction and modification of DNA in Escherichia coli.
TL;DR: Intercistronic complementation was observed between three classes of restriction and modification mutants of E. coli B, indicating that at least three cistron (the ram cistrons) are involved in the genetic control of the [restriction and modification of DNA].
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Codon--anticodon pairing: the wobble hypothesis.
TL;DR: In this paper, it is suggested that while the standard base pairs may be used rather strictly in the first two positions of the triplet, there may be some wobble in the pairing of the third base.
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The Isolation and Characterization of Linked δ- and β-Globin Genes from a Cloned Library of Human DNA
TL;DR: The two independently isolated β-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the δ- globin gene of one of the clones, which suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the PstI restriction enzyme recognition sequence.