An electrophoretic karyotype for yeast
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TLDR
With the possible exception of chromosomes that differ greatly in size or electrophoretic behavior from all the known chromosomes, the results appear to define a complete "electrophoretics karyotype" for yeast.Abstract:
The chromosomal DNA molecules of a standard laboratory strain of Saccharomyces cerevisiae have been separated into 12 well-resolved bands by orthogonal-field-alternation gel electrophoresis. DNA X DNA hybridization probes derived from cloned genes have been used to correlate this banding pattern with yeast's genetically defined chromosomes. The 12 bands are shown to represent 9 singlets and 3 comigrating doublets, thereby accounting for 15 chromosomes that were identified as I-XI and XIII-XVI. Because the three comigrating doublets could be readily resolved in certain laboratory yeast strains that contain chromosome-length polymorphisms relative to our standard strain, all 15 of these chromosomes could be displayed as a single band in at least one of four strains that were studied. A 16th chromosome (number XII), which is known to contain the genes for rRNA, does not reproducibly enter the gels. By making use of the band identifications, the previously unmapped fragment F8 was assigned to chromosome XIII. With the possible exception of chromosomes that differ greatly in size or electrophoretic behavior from all the known chromosomes, the results appear to define a complete "electrophoretic karyotype" for yeast.read more
Citations
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References
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Journal ArticleDOI
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis
TL;DR: This pulsed field gradient gel electrophoresis fractionates intact S. cerevisiae chromosomal DNA, producing a molecular karyotype that greatly facilitates the assignment of genes to yeast chromosomes.
Journal ArticleDOI
Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis
TL;DR: The design of the apparatus, the electrophoretic protocol, and the sample-handling procedures that are employed are described, and several of which have been shown by DNA-DNA hybridization to hybridize uniquely to different chromosome-specific hybridization probes are described.
Journal ArticleDOI
Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast
TL;DR: The mutants were tested for loss of viability, change in morphology, increase in cell number, and ability to synthesize protein, ribonucleic acid (RNA), and deoxyribonuclear acid (DNA) after a shift from 23 to 36 C as mentioned in this paper.
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The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide.
TL;DR: The mechanism of this conversion in Saccharomyces cerevisiae is investigated by examining the properties of mitochondrial DNA at various times during the mutation process, using cycloheximide to amplify the proportion of radioactivity in mitochondrial DNA.
Journal ArticleDOI
Genetic map of Saccharomyces cerevisiae.
R K Mortimer,D Schild +1 more
TL;DR: Although all of the data presented in this article are from tetrad analyses, many other techniques have been used to assign genes to chromosomes or to specific chromosome arms, including aneuploid analysis, mitotic recombination analysis,Mitotic chromosome loss or nondisjunction, and random spore analysis.