Journal ArticleDOI
Analysis of the complexity and diversity of mRNA from chicken liver and oviduct
TLDR
These studies demonstrate that 85% of the different mRNA sequences detected are present in both liver and oviduct, and suggest that the vast majority of the information expressed as mRNA is required for the maintenance of cellular functions common to all tissues.About:
This article is published in Cell.The article was published on 1976-02-01. It has received 165 citations till now. The article focuses on the topics: cDNA library & Oviduct.read more
Citations
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Purification and biochemical heterogeneity of the mammalian SWI-SNF complex.
Weidong Wang,Jacques Côté,Yutong Xue,Sharleen Zhou,Paul A. Khavari,Stephen R. Biggar,Christian Muchardt,Ganjam V. Kalpana,Stephen P. Goff,Moshe Yaniv,Jerry L. Workman,Gerald R. Crabtree +11 more
TL;DR: Purification of the SWI‐SNF2 homologs demonstrates that it is heterogeneous with respect to subunit composition, and certain cell lines completely lack BRG1 and hbrm, indicating that they are not essential for cell viability and that the mammalian SWI-SNF complex may be tailored to the needs of a differentiated cell type.
Journal ArticleDOI
Replication timing of genes and middle repetitive sequences
TL;DR: A change in the replication timing of a tissue-specific gene appears to reflect the commitment of that gene to transcriptional competence or to quiescence during ontogeny, consistent with a model in which two functionally distinct genomes coexist in the nucleus.
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Regulation of gene expression: possible role of repetitive sequences
Eric H. Davidson,Roy J. Britten +1 more
TL;DR: It is surprising that both complements of most repeat sequences are present in nuclear RNA, which leads to model for regulation of gene expression in which the formation of repetitive RNA-RNA duplexes controls the production of messenger RNA.
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The expression of three abundance classes of messenger RNA in mouse tissues
TL;DR: Tissue-specific differences in mRNA populations may be related to abundance as well as qualitative differences, and cross-hybridization experminets show that the most abundant class of mRNA in each tissue is characteristic, and that a high proportion of the total sequences are common between tissues.
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Production of mRNA in chinese hamster cells: Relationship of the rate of synthesis to the cytoplasmic concentration of nine specific mRNA sequences
TL;DR: Cloned DNA sequences complementary to unselected mRNAs from Chinese hamster ovary cells are constructed and used in RNA:DNA hybridization experiments, suggesting the synthesis of many hnRNA molecules that are qualitatively different from those which eventually contribute mRNA to the cytoplasm.
References
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Journal ArticleDOI
Three abundance classes in HeLa cell messenger RNA
TL;DR: Approximately 35,000 different poly(A)-containing RNA sequences are present in HeLa cell cytoplasm and the sequences are grouped in three distinct abundance classes.
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The metabolism of a poly(A) minus mRNA fraction in HeLa cells
TL;DR: The data indicate that poly(A) − mRNA is not short-lived nuclear or cytoplasmic heterogeneous RNA contamination, and that the half-life of the poly( a) − RNA may parallel that of thePoly(A), + mRNA, and the method of RNA fractionation precludes contamination.
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Identification and Isolation of Ovalbumin-synthesizing Polysomes I. SPECIFIC BINDING OF 125I-ANTI-OVALBUMIN TO POLYSOMES
TL;DR: Techniques were developed for the isolation of undegraded hen oviduct polysomes and for the preparation of 125I-γ-globulin free of ribonuclease activity to indicate that the reaction is specific for polysome that synthesize ovalbumin.
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Antagonism of cholera enterotoxin by anti-inflammatory agents in the rat.
Henry I. Jacoby,C. H. Marshall +1 more
TL;DR: A drug which could be given orally to reverse the massive loss of fluid and electrolytes by antagonizing the effect of cholera enterotoxin on secretory mechanisms of the small intestine has been suggested as a rational means to attack cholwater with the least number of trained people and equipment as discussed by the authors.
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Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid☆
J. O. Bishop,Michael Rosbash +1 more
TL;DR: It is concluded that if duck DNA does contain poly(dA) sequences corresponding to mRNA-associated poly(rA) sequence, there are fewer than 8000 of these per haploid genome.