Book ChapterDOI
Automated measurement and monitoring of bioprocesses: key elements of the M(3)C strategy.
Reads0
Chats0
TLDR
This chapter surveys the principles of monitoring rather than compiling instruments, and some new(er) instrumental analytical tools, interfaced to bioprocesses, are explained.Abstract:
The state-of-routine monitoring items established in the bioprocess industry as well as some important state-of-the-art methods are briefly described and the potential pitfalls discussed. Among those are physical and chemical variables such as temperature, pressure, weight, volume, mass and volumetric flow rates, pH, redox potential, gas partial pressures in the liquid and molar fractions in the gas phase, infrared spectral analysis of the liquid phase, and calorimetry over an entire reactor. Classical as well as new optical versions are addressed. Biomass and bio-activity monitoring (as opposed to "measurement") via turbidity, permittivity, in situ microscopy, and fluorescence are critically analyzed. Some new(er) instrumental analytical tools, interfaced to bioprocesses, are explained. Among those are chromatographic methods, mass spectrometry, flow and sequential injection analyses, field flow fractionation, capillary electrophoresis, and flow cytometry. This chapter surveys the principles of monitoring rather than compiling instruments.read more
Citations
More filters
Journal ArticleDOI
Translating Molecular Recognition into a Pressure Signal to enable Rapid, Sensitive, and Portable Biomedical Analysis
Zhi Zhu,Zhichao Guan,Dan Liu,Shasha Jia,Jiuxing Li,Zhi-Chao Lei,Shuichao Lin,Tianhai Ji,Zhong-Qun Tian,Chaoyong James Yang +9 more
TL;DR: A very familiar, yet underutilized, physical parameter—gas pressure—can serve as signal readout for highly sensitive bioanalysis and opens up a new way for simple, portable, yet highly sensitive biomedical analysis in a variety of settings.
Journal ArticleDOI
Sensor systems for bioprocess monitoring
TL;DR: In this paper, the authors provide an overview of current and emerging sensors for bioprocess monitoring and control, including sensors directly interfaced to bioreactors for measuring important variables from the gas phase, such as oxygen and carbon dioxide concentration, and sensors for the monitoring of the biomass concentration and morphology and of the changing medium composition.
Journal ArticleDOI
Distance-based microfluidic quantitative detection methods for point-of-care testing
TL;DR: This review summarizes the main methods for translation of molecular signals to distance-based readout and discusses different microfluidic platforms in terms of applications in biomedical diagnostics, food safety monitoring, and environmental analysis.
Journal ArticleDOI
Microbial heterogeneity affects bioprocess robustness: dynamic single-cell analysis contributes to understanding of microbial populations.
Frank Delvigne,Philippe Goffin +1 more
TL;DR: Although microbial phenotypic heterogeneity has been thoroughly investigated at a fundamental level, the implications of this phenomenon in the context of microbial bioprocesses are still subject to debate and automated flow cytometry is the best technique for investigating microbial heterogeneity under process conditions.
Journal ArticleDOI
A review of control strategies for manipulating the feed rate in fed-batch fermentation processes.
TL;DR: This review covers a range of strategies employed to use the feed rate as a manipulated variable in a control strategy, as it is seen that this variable may be used towards many different objectives depending on the process of interest, the characteristics of the strain, or the product being produced, which leads to different drivers for process optimisation.
References
More filters
Journal ArticleDOI
Electronic nose for estimation of product concentration in mammalian cell cultivation
TL;DR: It is shown that the electronic nose in combination with an artificial neural network can be used for on-line estimation of the Factor VIII concentration in production-scale cultivations, and this non-invasive method is potentially useful foron-line bioprocess monitoring.
Journal ArticleDOI
Optische Inline-Messverfahren zur Zellzahl- und Zellgrößenbestimmung in der Bioprozesstechnik
TL;DR: In this article, a new optische Verfahren vorgestellt, in which the Inline bestimmung of Zellzahl, Zellmorphology and Zellgrosenverteilung is eingesetzt.
Journal ArticleDOI
Online Spectroscopic Investigations (FTIR/Raman) of Industrial Reactions: Synthesis of Tributyltin Azide and Hydrogenation of Chloronitrobenzene
Jacques Wiss,Arne Zilian +1 more
TL;DR: In this paper, the utilization of online spectroscopic methods (FTIR/Raman) for the development and the monitoring of chemical processes was demonstrated with two important chemical reaction types.
Journal ArticleDOI
Fermentation reactor coupled with capillary electrophoresis for on-line bioprocess monitoring.
TL;DR: A filter probe integrated into a computerized pneumatic sampler for capillary electroseparations was developed for an on-line monitoring of bioprocesses and the resolution was found to be the most important parameter influencing the performance of the microbial metabolism.
Journal ArticleDOI
Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis
TL;DR: A simple and robust analyser system based on sequential injection analysis for the determination of sorbitol in Pichia pastoris MutS phenotype batch fermentation is described and proved to be reproducible.