Journal ArticleDOI
Bioreactor monitoring with spectroscopy and chemometrics: a review
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TLDR
In this review, published material on the potential application of different spectroscopic techniques for bioreactor monitoring is critically discussed, with particular emphasis on optical fiber technology, reported for in situ bioprocess monitoring.Abstract:
Biotechnological processes are crucial to the development of any economy striving to ensure a relevant position in future markets. The cultivation of microorganisms in bioreactors is one of the most important unit operations of biotechnological processes, and real-time monitoring of bioreactors is essential for effective bioprocess control. In this review, published material on the potential application of different spectroscopic techniques for bioreactor monitoring is critically discussed, with particular emphasis on optical fiber technology, reported for in situ bioprocess monitoring. Application examples are presented by spectroscopy type, specifically focusing on ultraviolet–visible, near-infrared, mid-infrared, Raman, and fluorescence spectroscopy. The spectra acquisition devices available and the major advantages and disadvantages of each spectroscopy are discussed. The type of information contained in the spectra and the available chemometric methods for extracting that information are also addressed, including wavelength selection, spectra pre-processing, principal component analysis, and partial least-squares. Sample handling techniques (flow and sequential injection analysis) that include transport to spectroscopic sensors for ex-situ on-line monitoring are not covered in this review.read more
Citations
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Journal ArticleDOI
Raman spectroscopy as a process analytical technology for pharmaceutical manufacturing and bioprocessing
TL;DR: Raman spectroscopy has made a positive impact as a process analytical and control tool for pharmaceutical manufacturing and bioprocessing, with demonstrated scientific and financial benefits throughout a product’s lifecycle.
Journal ArticleDOI
Sensor systems for bioprocess monitoring
TL;DR: In this paper, the authors provide an overview of current and emerging sensors for bioprocess monitoring and control, including sensors directly interfaced to bioreactors for measuring important variables from the gas phase, such as oxygen and carbon dioxide concentration, and sensors for the monitoring of the biomass concentration and morphology and of the changing medium composition.
Journal ArticleDOI
Emerging non-destructive terahertz spectroscopic imaging technique: Principle and applications in the agri-food industry
TL;DR: It is expected that THz technique combined with chemometric methods has great potential for agri-food products evaluation, and continuous wave THz imaging performs better for identifying low-density foreign bodies embedded in foodstuffs as compared to X-ray imaging.
Journal ArticleDOI
Microbial heterogeneity affects bioprocess robustness: dynamic single-cell analysis contributes to understanding of microbial populations.
Frank Delvigne,Philippe Goffin +1 more
TL;DR: Although microbial phenotypic heterogeneity has been thoroughly investigated at a fundamental level, the implications of this phenomenon in the context of microbial bioprocesses are still subject to debate and automated flow cytometry is the best technique for investigating microbial heterogeneity under process conditions.
Journal ArticleDOI
Spectroscopic sensors for in-line bioprocess monitoring in research and pharmaceutical industrial application
TL;DR: A critical overview of the most common in-line spectroscopic techniques is given and examples are provided of the wide range of possible applications in upstream processing and downstream processing of spectroscopes for real-time monitoring to optimize productivity and ensure product quality in the pharmaceutical industry.
References
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Proceedings ArticleDOI
Fuzzy clustering with a fuzzy covariance matrix
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