Journal ArticleDOI
Biomass measurement online: the performance of in situ measurements and software sensors
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TLDR
This review concentrates on the applications of dielectric spectroscopy, optical density, infrared spectroscopic, and fluorescence for in situ measurement of biomass.Abstract:
Biomass measurement is one of the most critical measurements in biotechnological processes. The technologies developed for the measurement of biomass in situ have developed over the years. Because it has been over 10 years since the last review concentrating on practical issues concerning biomass measurements, it is time to evaluate recent developments in the field. This review concentrates on the applications of dielectric spectroscopy, optical density, infrared spectroscopy, and fluorescence for in situ measurement of biomass. The advantages offered by these methods and an economic way of estimating biomass concentration, the software sensors, are considered.read more
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Assessment of Recent Process Analytical Technology (PAT) Trends: A Multiauthor Review
Levente L. Simon,Hajnalka Pataki,György Marosi,Fabian Meemken,Konrad Hungerbühler,Alfons Baiker,Srinivas Tummala,Brian Glennon,Martin Kuentz,Gerry Steele,Herman J. M. Kramer,James W. Rydzak,Zeng-Ping Chen,Julian Morris,Francois Kjell,Ravendra Singh,Rafiqul Gani,Krist V. Gernaey,Marjatta Louhi-Kultanen,John Oreilly,Niklas Sandler,Osmo Antikainen,Jouko Yliruusi,Patrick Frohberg,Joachim Ulrich,Richard D. Braatz,Tom Leyssens,Moritz von Stosch,Rui Oliveira,Reginald B. H. Tan,Huiquan Wu,Mansoor A. Khan,Des O'grady,Anjan Pandey,Remko Westra,Emmanuel Delle-Case,Detlef Pape,Daniele Angelosante,Yannick Maret,Olivier Steiger,Miklos Lenner,Kaoutar Abbou-Oucherif,Zoltan K. Nagy,James D. Litster,Vamsi Krishna Kamaraju,Min-Sen Chiu +45 more
TL;DR: In this paper, a multiauthor review article aims to bring readers up to date with some of the current trends in the field of process analytical technology (PAT) by summarizing each aspect of the subject (sensor development, PAT based process monitoring and control methods) and presenting applications both in industrial laboratories and in manufacture.
Journal ArticleDOI
Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: A review
TL;DR: Several bioprocess engineering aspects related to P. pastoris cultivation are discussed, including the different promoters available, both constitutive and inductive, on- and off-line process parameter monitoring methods, fed-batch and continuous cultivation control strategies, proteolytic degradation of products and methods to minimize associated yield reductions, and the different models devised to describe cell growth and protein production.
Journal ArticleDOI
Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: A review.
TL;DR: Large-scale production of proteins via high cell density fermentation additionally relies on the optimization of process parameters including methanol feed rate, induction temperature and specific growth rate, so P. pastoris is becoming an indispensable platform through the use of combined engineering strategies.
Journal ArticleDOI
Soft sensors in bioprocessing: A status report and recommendations
Reiner Luttmann,Daniel G. Bracewell,Gesine Cornelissen,Krist V. Gernaey,Jarka Glassey,Volker C. Hass,Christian M. Kaiser,Christian Preusse,Gerald Striedner,Carl-Fredrik Mandenius +9 more
TL;DR: The panel was to provide an update on the present status of the subject and to identify critical needs and issues for the furthering of the successful development of soft sensor methods in bioprocess engineering research and for industrial applications, in particular with focus on biopharmaceutical applications.
Journal ArticleDOI
Dielectric spectroscopy as a viable biosensing tool for cell and tissue characterization and analysis.
TL;DR: This review covers the different methods of interpreting dielectric spectra and progress made in applications of impedance spectroscopy for cell observations, which include observing cell differentiation, mitosis and the cell cycle and cytotoxicity/cell death.
References
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Erklärung der dielektrischen Nachwirkungsvorgänge auf Grund Maxwellscher Vorstellungen
Book
Encyclopedia of bioprocess technology : fermentation, biocatalysis, and bioseparation
TL;DR: The five--volume set of the Encyclopedia of Bioprocess Technology presents the applications and established theories in biotechnology--focusing on industrial applications of fermentation, biocatalysis and bioseparation.
Journal ArticleDOI
The dielectric properties of biological cells at radiofrequencies : Applications in biotechnology
TL;DR: The dielectric properties of cells and their components and the electrical techniques that use them will be discussed mainly in the context of biotechnology but some applications in medicine will also be highlighted.
Journal ArticleDOI
Two-Dimensional Fluorescence Spectroscopy: A New Tool for On-Line Bioprocess Monitoring
TL;DR: Two‐dimensional fluorescence spectroscopy is presented as a new method for bioprocess monitoring that covers a wide range of excitation and emission wavelengths and is a further development of the fluorescence measurements performed so far, which concentrated mainly on NAD(P) H culture fluorescence.
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