BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradation
TLDR
It is shown that BBX21 binds to the T/G-box in the ELONGATED HYPOCOTYL 5 (HY5) promoter and directly activates HY5 expression in the light, indicating thatBBX21 is a pivotal component involved in the COP1-HY5 regulatory hub.Abstract:
BBX21 (also known as SALT TOLERANCE HOMOLOG 2), a B-box (BBX)-containing protein, has been previously identified as a positive regulator of light signaling; however, the precise role of BBX21 in regulating seedling photomorphogenesis remains largely unclear. In this study, we report that CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) interacts with BBX21 in vivo and is able to ubiquitinate BBX21 in vitro. Thus, BBX21 is targeted for 26S proteasome-mediated degradation in dark-grown Arabidopsis seedlings in a COP1-dependent manner. Moreover, we show that BBX21 binds to the T/G-box in the ELONGATED HYPOCOTYL 5 (HY5) promoter and directly activates HY5 expression in the light. Transgenic seedlings overexpressing BBX21 exhibit dramatically shortened hypocotyls in the light, and this phenotype is dependent on a functional HY5. Taken together, our data suggest a molecular base underlying BBX21-mediated seedling photomorphogenesis, indicating that BBX21 is a pivotal component involved in the COP1-HY5 regulatory hub.read more
Citations
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The Multifaceted Roles of HY5 in Plant Growth and Development
TL;DR: Recent advances on HY5 research in diverse aspects of plant development are reviewed and still open questions that need to be addressed in the near future for a complete understanding of its function in plant signaling and beyond are highlighted.
Journal ArticleDOI
Photoreceptor-mediated regulation of the COP1/SPA E3 ubiquitin ligase.
Roman Podolec,Roman Ulm +1 more
TL;DR: This work has shown that the direct interaction of light-activated photoreceptors with the COP1/SPA complex represses its activity via nuclear exclusion of COP1, disruption of the Cop1-SPA interaction, and/or SPA protein degradation, which enables plants to integrate different light signals at the level of the COP 1/SPAcomplex to enact appropriate photomorphogenic responses according to the light environment.
Journal ArticleDOI
BBX16, a B-box protein, positively regulates light-induced anthocyanin accumulation by activating MYB10 in red pear.
Songling Bai,Ruiyan Tao,Yinxin Tang,Lei Yin,Yunjing Ma,Junbei Ni,Xinhui Yan,Qinsong Yang,Zhongying Wu,Yanling Zeng,Yuanwen Teng +10 more
TL;DR: This work uncovered regulatory modes for PpBBX16 and suggested the potential functions of other pear BBX genes in the regulation of anthocyanin accumulation, thereby providing target genes for further studies on anthocianin biosynthesis.
Journal ArticleDOI
Plant Strategies for Enhancing Access to Sunlight.
TL;DR: The photoperception mechanisms and the growth responses elicited by the neighboring vegetation in shade-avoiding plants are described, focusing on Arabidopsis thaliana, where these responses are best understood.
Journal ArticleDOI
A PIF1/PIF3-HY5-BBX23 Transcription Factor Cascade Affects Photomorphogenesis
TL;DR: This study reveals that the transcriptional cascade consisting of PIF1/PIF3, HY5, and BBX23 controls photomorphogenesis, providing a transcriptional regulatory layer by which plants fine-tune their growth in response to changing light environment.
References
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TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
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TL;DR: The utility of pEarleyGate destination vectors for the expression of epitope-tagged proteins that can be affinity captured or localized by immunofluorescence microscopy is demonstrated.
Journal ArticleDOI
Targeted destabilization of HY5 during light-regulated development of Arabidopsis
TL;DR: It is shown that the abundance of HY5 is directly correlated with the extent of photomorphogenic development, and that the COP1–HY5 interaction may specifically target HY5 for proteasome-mediated degradation in the nucleus.
Journal ArticleDOI
Analysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of Development
Jungeun Lee,Jungeun Lee,Kun He,Viktor Stolc,Viktor Stolc,Horim Lee,Pablo Figueroa,Ying Gao,Ying Gao,Waraporn Tongprasit,Hongyu Zhao,Ilha Lee,Xing Wang Deng,Xing Wang Deng +13 more
TL;DR: It is demonstrated that in vivo association of HY5 with promoter targets is not altered under distinct light qualities or during light-to-dark transition, and a model in which HY5 is a high hierarchical regulator of the transcriptional cascades for photomorphogenesis is supported.
Journal ArticleDOI
The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus-induced development of root and hypocotyl
TL;DR: P phenotypes indicate that the HY5 gene is responsible for the regulation of fundamental developmental processes of the plant cell: cell elongation, cell proliferation, and chloroplast development.