Beam-induced motion of vitrified specimen on holey carbon film.
Axel F. Brilot,James Z. Chen,James Z. Chen,Anchi Cheng,Junhua Pan,Stephen C. Harrison,Stephen C. Harrison,Clinton S. Potter,Bridget Carragher,Richard Henderson,Nikolaus Grigorieff,Nikolaus Grigorieff +11 more
TLDR
This work studies the amount and direction of motion of virus particles suspended in thin vitrified ice layers across holes in perforated carbon films using exposure series and shows how alignment and averaging of movie frames can be used to restore high-resolution detail in images affected by beam-induced motion.About:
This article is published in Journal of Structural Biology.The article was published on 2012-03-01 and is currently open access. It has received 368 citations till now. The article focuses on the topics: Biological specimen & Magnetosphere particle motion.read more
Citations
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Journal ArticleDOI
MotionCor2: Anisotropic Correction of Beam-Induced Motion for Improved Cryo-Electron Microscopy
Shawn Q. Zheng,Eugene Palovcak,Jean Paul Armache,Kliment A. Verba,Yifan Cheng,David A. Agard +5 more
TL;DR: MotionCor2 software corrects for beam-induced sample motion, improving the resolution of cryo-EM reconstructions.
Journal ArticleDOI
RELION: implementation of a Bayesian approach to cryo-EM structure determination.
TL;DR: Developments that reduce the computational costs of the underlying maximum a posteriori (MAP) algorithm, as well as statistical considerations that yield new insights into the accuracy with which the relative orientations of individual particles may be determined are described.
Journal ArticleDOI
New tools for automated high-resolution cryo-EM structure determination in RELION-3.
Jasenko Zivanov,Takanori Nakane,Björn O. Forsberg,Dari Kimanius,Wim J. H. Hagen,Erik Lindahl,Erik Lindahl,Sjors H.W. Scheres +7 more
TL;DR: CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations in the third major release of RELION.
Journal ArticleDOI
Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM
Xueming Li,Paul Mooney,Shawn Q. Zheng,Shawn Q. Zheng,Christopher R. Booth,Michael B. Braunfeld,Michael B. Braunfeld,Sander Gubbens,David A. Agard,David A. Agard,Yifan Cheng +10 more
TL;DR: This approach determined a 3.3-Å-resolution structure of an ∼700-kDa protein with D7 symmetry, the Thermoplasma acidophilum 20S proteasome, showing clear side-chain density and greatly enhances image quality and data acquisition efficiency.
Journal ArticleDOI
How cryo-EM is revolutionizing structural biology
TL;DR: The recent advances in electron detection and image processing are reviewed and the exciting new opportunities that they offer to structural biology research are illustrated.
References
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EMAN2: an extensible image processing suite for electron microscopy.
Guang Tang,Liwei Peng,Philip R. Baldwin,Deepinder S. Mann,Wen Jiang,I. Rees,Steven J. Ludtke +6 more
TL;DR: EMAN2 has been under development for the last two years, with a completely refactored image processing library, and a wide range of features to make it much more flexible and extensible than EMAN1.
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Spider and web : processing and visualization of images in 3d electron microscopy and related fields
Joachim Frank,Michael Radermacher,Michael Radermacher,Pawel A. Penczek,Jun Zhu,Jun Zhu,Yanhong Li,Mahieddine Ladjadj,Ardean Leith +8 more
TL;DR: Novel features are a suite of operations relating to the determination, modeling, and correction of the contrast transfer function and the availability of the entire documentation in hypertext format.
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Accurate determination of local defocus and specimen tilt in electron microscopy
TL;DR: Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.
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The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules.
TL;DR: Because of the lack of sufficiently bright neutron sources in the foreseeable future, electron microscopy in practice provides the greatest potential for immediate progress.
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MRC Image Processing Programs
TL;DR: This paper describes the current state of a large set of programs written by various members of the Laboratory of Molecular Biology for processing images of two-dimensional crystals and of particles with helical or icosahedral symmetry for determination of macromolecular structures by electron microscopy.