Binding of the La autoantigen to the hepatitis C virus 3′ untranslated region protects the RNA from rapid degradation in vitro
TLDR
It is found that the 3' end of positive polarity HCV RNA is sensitive to cytosolic RNases whereas the 3', untranslated region with the cellular La protein prevented premature degradation of the HCV RNAs.Abstract:
We have analysed hepatitis C virus (HCV) RNAs in an in vitro RNA degradation assay. We found that the 3′ end of positive polarity HCV RNA is sensitive to cytosolic RNases whereas the 3′ end of negative polarity HCV RNA is relatively stable. Interaction of the HCV 3′ untranslated region with the cellular La protein prevented premature degradation of the HCV RNA. One may speculate that HCV RNAs interact with La protein in infected cells to prevent premature degradation of the viral RNAs.read more
Citations
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Journal ArticleDOI
The la protein
Sandra L. Wolin,Tommy Cedervall +1 more
TL;DR: This review focuses on the roles of the Laprotein in small RNA biogenesis and also discusses data that implicate the La protein in the translation of specific mRNAs.
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Genetic Analysis of Sequences in the 3′ Nontranslated Region of Hepatitis C Virus That Are Important for RNA Replication
Peter Friebe,Ralf Bartenschlager +1 more
TL;DR: These data represent the first detailed mapping of sequences in the 3′ NTR assumed to act as a promoter for initiation of minus-strand RNA synthesis and demonstrate that this region is most crucial for efficient RNA replication.
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A cis-Acting Replication Element in the Sequence Encoding the NS5B RNA-Dependent RNA Polymerase Is Required for Hepatitis C Virus RNA Replication
TL;DR: Understanding the role of 5BSL3.2 and determining how this new CRE functions in the context of previously identified elements at the 5′ and 3′ ends of the RNA genome should provide new insights into HCV RNA replication.
Journal ArticleDOI
Translation Elongation Factor-1α, La, and PTB Interact with the 3′ Untranslated Region of Dengue 4 Virus RNA
TL;DR: The specific binding of La and PTB to the sequences considered essential for viral RNA replication may suggest that these proteins could function as RNA chaperones to maintain RNA structure in a conformation that favors viral replication, while EF-1alpha may function as an RNA helicase.
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La autoantigen is necessary for optimal function of the poliovirus and hepatitis C virus internal ribosome entry site in vivo and in vitro.
TL;DR: It is demonstrated that depletion of La causes a dramatic reduction in poliovirus IRES function in vivo and a function of the La autoantigen in IRES-dependent translation is provided, which is defined as the step of translation which is stimulated by La.
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