Journal ArticleDOI
Bladder smooth muscle cells interaction and proliferation on PCL/PLLA electrospun nanofibrous scaffold.
Nasser Shakhssalim,Javad Rasouli,Reza Moghadasali,Farzaneh Sharifi Aghdas,Mohammad Naji,Masoud Soleimani +5 more
TLDR
Results showed that the novel porous nanofibrous electrospun scaffold is a biocompatible structure for attachment and adhesion of BdSMCs, however, there is not enough information on the stimulating effect of this nanofiber on the cells.Abstract:
PURPOSE Numerous synthetic materials have been used for the bladder reconstruction; of which, nano-structured scaffolds are used as relevant implant to the bladder tissue-engineering. The aim of this study was to investigate the capacity of Poly e-caprolactone/poly-L-lactide acid (PCL/PLLA) nanofibrous scaffold, in supporting the maintenance and attachment of the human bladder smooth muscle cells (BdSMCs). METHODS In this study, BdSMCs were isolated by enzymatic digestion method. Then, cells were seeded on PCL/PLLA nanofibrous scaffolds. Thereafter, cell attachment and expansion were analyzed by Hematoxylin and Eosin staining (H&E), immunohistochemistry, and scanning electron microscopy (SEM). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed that the nano-structured scaffold supported and maintained normal cell viability without inducing cytotoxic events. RESULTS H&E staining, immunohistochemistry, and SEM showed that BdSMCs were attached and expanded on PCL/PLLA nanofibrous scaffolds after 14 days. Cell viability of BdSMCs on PCL/PLLA nanofibrous scaffolds increased during 14 days. CONCLUSION Our results showed that the novel porous nanofibrous electrospun scaffold is a biocompatible structure for attachment and adhesion of BdSMCs. However, there is not enough information on the stimulating effect of this nanofiber on the cells. Therefore, further in-vivo studies seem required to confirm such a nanofiber to be used in the bladder tissue-engineering.read more
Citations
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Journal ArticleDOI
Biomatrices for bladder reconstruction.
Hsueh Kung Lin,Sundararajan V. Madihally,Blake W. Palmer,Dominic Frimberger,Kar Ming Fung,Bradley P. Kropp +5 more
TL;DR: For functional bladder regeneration, procedures for biomaterial fabrication, incorporation of biologically active agents, introduction of nanotechnology, and application of stem-cell technology need to be standardized.
Journal ArticleDOI
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?
Marta Pokrywczyńska,Arkadiusz Jundzill,Jan Adamowicz,Tomasz Kowalczyk,Karolina Warda,Marta Rasmus,Lukasz Buchholz,Sandra Krzyzanowska,Paweł Nakielski,Tomasz Chmielewski,Magdalena Bodnar,Andrzej Marszałek,Robert Dębski,Małgorzata Frontczak-Baniewicz,Grzegorz Mikułowski,Maciej Nowacki,Tomasz Kowalewski,Tomasz Drewa +17 more
TL;DR: The new five-layered poly (L–lactide–co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.
Journal ArticleDOI
Tissue Engineering in Pediatric Bladder Reconstruction-The Road to Success.
TL;DR: Major achievements and challenges in bladder tissue regeneration are discussed with a focus on different strategies to overcome the obstacles and to meet the need for living functional tissue replacements with a good growth potential and a long life span matching the pediatric population.
Journal ArticleDOI
The Current Use of Stem Cells in Bladder Tissue Regeneration and Bioengineering
TL;DR: This review presents the recent advancements in the use of stem cells in bladder tissue bioengineering and discusses their applications in tissue regeneration and bioengineering.
Journal ArticleDOI
Bladder smooth muscle cells on electrospun poly(ε-caprolactone)/poly(l-lactic acid) scaffold promote bladder regeneration in a canine model
Nasser Shakhssalim,Masoud Soleimani,Mohammad Mehdi Dehghan,Javad Rasouli,Masoud Taghizadeh-Jahed,Peyman Mohammadi Torbati,Mohammad Naji +6 more
TL;DR: Electrospun PCL/PLLA scaffold with proper handling, suture retention, nano-sized surface features, maintenance of normal phenotype of cells and minimal adverse effects in body can be a supportive substrate for bladder wall regeneration when seeded with bladder smooth muscle cells.
References
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Electrospinning of collagen nanofibers.
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Fabrication of nano-structured porous PLLA scaffold intended for nerve tissue engineering
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Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro.
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Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor.
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