Journal ArticleDOI
BMP-2 Derived Peptide and Dexamethasone Incorporated Mesoporous Silica Nanoparticles for Enhanced Osteogenic Differentiation of Bone Mesenchymal Stem Cells
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TLDR
Collectively, the BMP-2 peptide and DEX incorporated MSNs can act synergistically to enhance osteogenic differentiation of BMSCs, which have potential applications in bone tissue engineering.Abstract:
Bone morphogenetic protein-2 (BMP-2), a growth factor that induces osteoblast differentiation and promotes bone regeneration, has been extensively investigated in bone tissue engineering. The peptides of bioactive domains, corresponding to residues 73-92 of BMP-2 become an alternative to reduce adverse side effects caused by the use of high doses of BMP-2 protein. In this study, BMP-2 peptide functionalized mesoporous silica nanoparticles (MSNs-pep) were synthesized by covalently grafting BMP-2 peptide on the surface of nanoparticles via an aminosilane linker, and dexamethasone (DEX) was then loaded into the channel of MSNs to construct nanoparticulate osteogenic delivery systems (DEX@MSNs-pep). The in vitro cell viability of MSNs-pep was tested with bone mesenchymal stem cells (BMSCs) exposure to different particle concentrations, revealing that the functionalized MSNs had better cytocompatibility than their bare counterparts, and the cellular uptake efficiency of MSNs-pep was remarkably larger than that of bare MSNs. The in vitro results also show that the MSNs-pep promoted osteogenic differentiation of BMSCs in terms of the levels of alkaline phosphatase (ALP) activity, calcium deposition, and expression of bone-related protein. Moreover, the osteogenic differentiation of BMSCs can be further enhanced by incorporating of DEX into MSNs-pep. After intramuscular implantation in rats for 3 weeks, the computed tomography (CT) images and histological examination indicate that this nanoparticulate osteogenic delivery system induces effective osteoblast differentiation and bone regeneration in vivo. Collectively, the BMP-2 peptide and DEX incorporated MSNs can act synergistically to enhance osteogenic differentiation of BMSCs, which have potential applications in bone tissue engineering.read more
Citations
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Journal ArticleDOI
Mesoporous Silica Nanoparticles: A Comprehensive Review on Synthesis and Recent Advances
TL;DR: The synthesis of mesoporous silica nanoparticles and the factors influencing the size and morphology of this wonder carrier are discussed.
Journal ArticleDOI
Mesoporous silica nanoparticles for therapeutic/diagnostic applications.
Samira Jafari,Hossein Derakhshankhah,Loghman Alaei,Ali Fattahi,Behrang Shiri Varnamkhasti,Ali Akbar Saboury +5 more
TL;DR: The common approaches of cargo loading and release processes from MSNs, the intracellular uptake, safety and cytotoxicity aspects of MSNs are discussed as well.
Journal ArticleDOI
Novel biomaterial strategies for controlled growth factor delivery for biomedical applications
TL;DR: This review evaluates the latest techniques in direct immobilization and relevant biomaterials used for GF loading and release, including synthetic polymers, albumin, polysaccharides, lipids, mesoporous silica-based nanoparticles (NPs), and polymeric capsules and focuses on GF-encapsulated NPs in functionalized microporous scaffolds as a promising alternative.
Journal ArticleDOI
Gold nanoparticle size and shape influence on osteogenesis of mesenchymal stem cells.
Jingchao Li,Jia'En Jasmine Li,Jing Zhang,Jing Zhang,Xinlong Wang,Xinlong Wang,Naoki Kawazoe,Guoping Chen,Guoping Chen +8 more
TL;DR: It was found that the size and shape of AuNPs affected the osteogenic differentiation of hMSCs through regulating the activation of Yes-associated protein (YAP) and should provide useful guidance for the preparation of Au NPs with defined size andshape for their biomedical applications.
Journal ArticleDOI
Electrospun Nanofibers for Tissue Engineering with Drug Loading and Release
TL;DR: In these studies, in vitro and in vivo experiments demonstrated that electrospun nanofibrous scaffolds exhibited desirable effects for the repair and treatment of damaged tissue and, thus, have excellent potential for clinical application.
References
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Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
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Journal ArticleDOI
Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers
TL;DR: This review highlights the recent research developments of a series of surface-functionalized mesoporous silica nanoparticle (MSN) materials as efficient drug delivery carriers and envision that these MSN-based systems have a great potential for a variety of drug delivery applications.
Journal ArticleDOI
Increased bone formation in osteocalcin-deficient mice
Patricia Ducy,Christelle Desbois,Brendan F. Boyce,Gerald J. Pinero,Beryl Story,Colin R. Dunstan,Erica Smith,Jeffrey Bonadio,Steven A. Goldstein,Caren M. Gundberg,Allan Bradley,Gerard Karsenty +11 more
TL;DR: This study provides the first evidence that osteocalcin is a determinant of bone formation, and generates osteocalin-deficient mice that develop a phenotype marked by higher bone mass and bones of improved functional quality.
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Mesoporous silica nanoparticles for drug delivery and biosensing applications
TL;DR: Recent research progress on the design of functional MSN materials with various mechanisms of controlled release, along with the ability to achieve zero release in the absence of stimuli, and the introduction of new characteristics to enable the use of nonselective molecules as screens for the construction of highly selective sensor systems are reviewed.
Journal ArticleDOI
The Osteoblast: A Sophisticated Fibroblast under Central Surveillance
TL;DR: A regulatory loop, which involves the hormone leptin, may help to explain the protective effect of obesity on bone mass in humans and provide a novel physiologic concept that may shed light on the etiology of osteoporosis and help to identify new therapeutic targets.