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Cassava brown streak disease: a threat to food security in Africa.

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TLDR
This review aims to provide a summary of the most important studies on the aetiology, epidemiology and control of CBSD and to highlight key research areas that need prioritization.
Abstract
Cassava brown streak disease (CBSD) has emerged as the most important viral disease of cassava (Manihot esculenta) in Africa and is a major threat to food security. CBSD is caused by two distinct species of ipomoviruses, Cassava brown streak virus and Ugandan cassava brown streak virus, belonging to the family Potyviridae. Previously, CBSD was reported only from the coastal lowlands of East Africa, but recently it has begun to spread as an epidemic throughout the Great Lakes region of East and Central Africa. This new spread represents a major threat to the cassava-growing regions of West Africa. CBSD-resistant cassava cultivars are being developed through breeding, and transgenic RNA interference-derived field resistance to CBSD has also been demonstrated. This review aims to provide a summary of the most important studies on the aetiology, epidemiology and control of CBSD and to highlight key research areas that need prioritization.

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Citations
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Journal ArticleDOI

Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence

TL;DR: The ability to modify multiple genes simultaneously in cassava to achieve tolerance to CBSD is demonstrated and this knowledge will be translated into an optimized strategy for protecting cassava from disease.
Journal ArticleDOI

Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses.

TL;DR: This study shows how combining knowledge on pathogen susceptibility factors with precise genome‐editing technologies offers a feasible solution for engineering transgene‐free genetic resistance in plants, even across species barriers.
Journal ArticleDOI

Gene expression atlas for the food security crop cassava

TL;DR: In this article, the authors provide molecular identities for 11 cassava tissue/organ types through RNA-sequencing and develop an open access, web-based interface for further interrogation of the data.
Journal ArticleDOI

Cassava brown streak disease: historical timeline, current knowledge and future prospects

TL;DR: Insight is provided into current CBSD knowledge, management efforts and future prospects for improved understanding needed to underpin effective control and mitigation of impacts on food security.
References
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Journal ArticleDOI

RNA virus error catastrophe: direct molecular test by using ribavirin.

TL;DR: It is demonstrated that ribavirin's antiviral activity is exerted directly through lethal mutagenesis of the viral genetic material, suggesting that RNA virus mutagens may represent a promising new class of antiviral drugs.
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An overlapping essential gene in the Potyviridae.

TL;DR: This work reports the discovery of a short ORF embedded within the P3 cistron of the polyprotein but translated in the +2 reading-frame, which suggests that other short overlapping genes may remain hidden even in well studied virus genomes and demonstrates the utility of the software package MLOGD as a tool for identifying such genes.
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Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.

TL;DR: Contigs were assembled from sequenced total siRNA from plants using small sequence assembly software and could positively identify RNA, ssDNA and dsDNA reverse transcribing viruses and in one case spanned the entire genome.
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