Journal ArticleDOI
Cell-mediated immune response and cross-protective efficacy of binary ethylenimine-inactivated bluetongue virus serotype-1 vaccine in sheep.
Channakeshava Sokke Umeshappa,Karam Pal Singh,Awadh Bihari Pandey,R. P. Singh,Roopa Hebbandi Nanjundappa +4 more
TLDR
The cross-protective efficacy of binary ethylenimine-inactivated BTV-1 vaccine was evaluated in Indian native sheep against virulent heterologous B TV-23 serotype challenge and suggested that inactivated B television vaccine induced appreciable cell-mediated immunity (CMI) and greatly reduced the severity of heterOLOGous B television-23 infection.About:
This article is published in Vaccine.The article was published on 2010-03-16. It has received 47 citations till now. The article focuses on the topics: Binary ethylenimine & Vaccination.read more
Citations
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Journal ArticleDOI
Multiserotype protection elicited by a combinatorial prime-boost vaccination strategy against bluetongue virus.
TL;DR: Examination of naked DNAs and recombinant modified vaccinia virus Ankara expressing VP2, VP7 and NS1 proteins from BTV-4 suggests that the DNA/rMVA-VP2,VP7,NS1 marker vaccine is a promising multiserotype vaccine against BTV.
Journal ArticleDOI
Characterization of protection afforded by a bivalent virus-like particle vaccine against Bluetongue Virus serotypes 1 and 4 in sheep
Ana Cristina Pérez de Diego,T.N. Athmaram,Meredith Stewart,Belén Rodríguez-Sánchez,José Manuel Sánchez-Vizcaíno,Rob Noad,Polly Roy +6 more
TL;DR: There is good evidence that BTV-1 VLPs delivered as monovalent or bivalent immunogen protect from bluetongue disease on challenge with virulent B TV-1, however, it is possible that there is some interference in protective response for Btv-4 in the bivalent BTV/4 VLP vaccine.
Journal ArticleDOI
T cell responses to bluetongue virus are directed against multiple and identical CD4+ and CD8+ T cell epitopes from the VP7 core protein in mouse and sheep.
TL;DR: Data demonstrate the activation of BTV-specific T cells during infection and vaccination and may provide an opportunity to develop DIVA-compliant vaccination approach to BTV encompassing a broad-spectrum of serotypes.
Journal ArticleDOI
Apoptosis and immuno-suppression in sheep infected with bluetongue virus serotype-23
Channakeshava Sokke Umeshappa,Karam Pal Singh,Roopa Hebbandi Nanjundappa,Awadh Bihari Pandey +3 more
TL;DR: In the present study, bluetongue virus (BTV)-induced apoptosis was studied in sheep blood and splenic mononuclear cells by analyzing annexin(+)-propidium iodide(-) early apoptotic cells, DNA ladder pattern, and caspase-3 gene expression to indicate involvement of BTV and IFN-alpha in the pathogenesis of BT.
Journal ArticleDOI
An updated review on bluetongue virus: epidemiology, pathobiology, and advances in diagnosis and control with special reference to India.
Mani Saminathan,Karam Pal Singh,Jaynudin Hajibhai Khorajiya,Murali Dinesh,S. Vineetha,Madhulina Maity,At Faslu Rahman,Jyoti Misri,Yashpal Singh Malik,Vivek Kumar Gupta,Raj Kumar Singh,Kuldeep Dhama +11 more
TL;DR: This review is the first to summarise the seroprevalence of BTV in India for 40 years, economic impact and pathobiology.
References
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Book ChapterDOI
Regulation of interferon-gamma during innate and adaptive immune responses.
TL;DR: The epigenetic modifications and three-dimensional structure of the Ifng locus in naive CD4 T cells, and the modifications they undergo as these cells differentiate into effector T cells suggest a model whereby the chromatin architecture of Ifng is poised to facilitate either rapid opening or silencing during Th1 or Th2 differentiation, respectively.
Journal ArticleDOI
An overview of real-time quantitative PCR: applications to quantify cytokine gene expression.
Annapaula Giulietti,Lutgart Overbergh,Dirk Valckx,Brigitte Decallonne,Roger Bouillon,Chantal Mathieu +5 more
TL;DR: The real-time RT-PCR technique is very accurate and sensitive, allows a high throughput, and can be performed on very small samples; therefore it is the method of choice for quantification of cytokine profiles in immune cells or inflamed tissues.
Journal ArticleDOI
Signals required for programming effector and memory development by CD8+ T cells.
Matthew F. Mescher,Julie M. Curtsinger,Pujya Agarwal,Kerry A. Casey,Michael Y. Gerner,Christopher D. Hammerbeck,Flavia E. Popescu,Zhengguo Xiao +7 more
TL;DR: Stimulation of naïve CD8+ T cells with antigen and costimulation results in proliferation and weak clonal expansion, but the cells fail to develop effector functions and are tolerant long term.
Journal ArticleDOI
Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes
TL;DR: It is found that murine cells infected with this virus were efficiently lysed in a major histocompatibility complex-restricted manner by cross-reactive CTL populations obtained by immunization with a variety of influenza A virus subtypes.
Influenza A virus nucleoprotein is a major target antigen for cross- reactive anti-influenza A virus cytotoxic T lymphocytes (vaccinia virus vector/influenza vaccine/internal viral protein/cell surface expression/cellular defense mechanism)
TL;DR: In this article, a recombinant vaccinia virus recombinant containing a DNA copy of an influenza A virus NP gene was constructed, and it was shown that the recombinant vac- cinia virus containing the PR8 NP gene is able to stimulate a vigorous secondary cross-reactive CTL response.