Characteristics of an immune system common to certain external secretions
Reads0
Chats0
TLDR
The γ1A present in saliva and colostrum exists largely in the form of higher polymers, the major component of which has a sedimentation coefficient of 11S, and its properties including the local production of a distinctive type of antibody separate it from the "systemic" system responsible for the production of circulating antibody.Abstract:
The γ1A present in saliva and colostrum exists largely in the form of higher polymers, the major component of which has a sedimentation coefficient of 11S. The 11S γ1A in these fluids differs from the polymers found in normal and myeloma sera both immunologically and by the fact that their sedimentation coefficients are unaffected by disulfide bond reduction in the absence of urea. However, like other γ-globulins the 11S γ1A molecules consist of multiple polypeptide chains linked by disulfide bonds. Local synthesis of γ1A in the salivary gland has been shown by fluorescent and autoradiographic studies, although the fraction of the total salivary γ1A which is derived from local production is uncertain. No evidence of transport of intravenously administered I131-labeled 7S γ1A from serum to saliva was obtained. Immunological specificity has been demonstrated in the salivary and colostral γ1A. Whether that portion of the γ1A which is immunologically specific is a piece incorporated during the local synthesis of γ1A in the gland or is added by the epithelial cell in the process of transport remains to be determined. Antibody activity (isohemagglutinins) have been demonstrated in saliva and colostrum and have been shown to be of the γ1A-type. In both of these fluids activity is associated primarily with γ1A-polymers of 11S and 18S sizes. There appears to be an immunological system which is characteristic of certain external secretions. Its properties including the local production of a distinctive type of antibody separate it from the "systemic" system responsible for the production of circulating antibody. This system may play a significant role in the body's defense mechanisms against allergens and microorganisms.read more
Citations
More filters
Journal ArticleDOI
Immunization against influenza in humans using an oral enteric-coated killed virus vaccine
Valerie Lazzell,Robert H. Waldman,Catherine Rose,Rashida Khakoo,Arthur I. Jacknowitz,Stephen A. Howard +5 more
TL;DR: These findings support the hypothesis that specialized collections of lymphoid cells in the small intestines have IgA precursor cells which circulate and populate distant mucosal sites and the oral method of influenza vaccination could prove to be superior in providing for immunological protection due to equal secretory antibody stimulation, improved convenience and less toxicity.
Book ChapterDOI
13 – Peyer's Patches as Inductive Sites for IgA Commitment
John J. Cebra,Khushroo E. Shroff +1 more
Journal ArticleDOI
Immunoglobulin Secretion in the Airways
TL;DR: The epithelial surface of the lung constitutes the next largest area that must be protected from invasion by microbes and toxic agents in the environment and two principal mechanisms have evolved for the surveillance and protection of this epithelial boundary.
Journal ArticleDOI
Antibacterial Activity of the Purified Peroxidase from Human Parotid Saliva
TL;DR: The peroxidase of human parotid saliva has been purified by concentration, gel filtration on Sephadex G-200, and ion exchange chromatography on Amberlite CG-50 and these studies support the involvement of the salivary per oxidase in an antibacterial system in saliva.
Journal ArticleDOI
Synthesis of secretory component by human colon cancer cells.
S.‐W. Huang,J. Fogh,R. Hong +2 more
TL;DR: In culture, two lines of carcinoma epithelium were shown to incorporate [14C]leucine into newly synthesized SC, which should provide useful preparations for studying the details of immunoglobulin‐SC interactions.
References
More filters
Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Efficient trace-labelling of proteins with iodine.
TL;DR: Values greater than 50 per cent can be obtained by adding oxidizing agents to liberate iodine from iodide, but most if not all of these appear to affect adversely the properties of the labelled protein.
Journal ArticleDOI
Localization of antigen in tissue cells ii. improvements in a method for the detection of antigen by means of fluorescent antibody
Albert H. Coons,Melvin H. Kaplan +1 more
TL;DR: Improvements in a method for the specific microscopic localization of antigen in tissue cells are described and two isomeric series derived from nitrofluorescein are described.
Related Papers (5)
Gamma-globulins: quantitative relationships in human serum and nonvascular fluids.
W. B. Chodirker,T. B. Tomasi +1 more