Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase.
Masayuki Otsu,Ian Hiles,Ivan Gout,Michael J. Fry,Fernanda Ruiz-Larrea,George Panayotou,Andrew J. Thompson,Ritu Dhand,J. Justin Hsuan,Nicholas F. Totty,Anthony D. Smith,Sarah J. Morgan,Sara A. Courtneidge,Peter J. Parker,Michael D. Waterfield +14 more
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The active PI3-kinase complex containing p85 alpha or p85 beta and the 110 kd protein binds to PDGF but not EGF receptors, suggesting that p85alpha and p85beta may mediate specific PI3 -kinase interactions with a subset of tyrosine kinases.About:
This article is published in Cell.The article was published on 1991-04-05 and is currently open access. It has received 739 citations till now. The article focuses on the topics: Receptor tyrosine kinase & Proto-oncogene tyrosine-protein kinase Src.read more
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Cellular functions regulated by src family kinases
Sheila M. Thomas,Joan S. Brugge +1 more
TL;DR: This chapter reviews the evidence implicating Src family kinases in specific receptor pathways and describes the mechanisms leading to their activation, the targets that interact with these kinases, and the biological events that they regulate.
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Rho GTPases and signaling networks
TL;DR: The Rho GTPases form a subgroup of the Ras superfamily of 20- to 30-kD GTP-binding proteins that have been shown to regulate a wide spectrum of cellular functions, and some of the more recent exciting findings hinting at novel, unanticipated functions of the RhoGTPases are summarized.
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Proteins regulating Ras and its relatives.
Mark S. Boguski,Frank McCormick +1 more
TL;DR: Many of these proteins are much larger and more complex than their targets, containing multiple domains capable of interacting with an intricate network of cellular enzymes and structures.
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SH2 and SH3 Domains: Elements that Control Interactions of Cytoplasmic Signaling Proteins
TL;DR: Observations suggest that SH2 and SH3 domains participate in the control of intracellular responses to growth factor stimulation.
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The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling
E. Lowenstein,Roger J. Daly,A. Batzer,W. Li,B. Margolis,Richard L. Lammers,Axel Ullrich,Edward Y. Skolnik,Dafna Bar-Sagi,Joseph Schlessinger +9 more
TL;DR: Results suggest that GRB2/sem-5 plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling.
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Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
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Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.