Journal ArticleDOI
Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method
TLDR
A simple, rapid method for retrieving cloned segments carrying a copy of the transposable element together with contiguous sequences corresponding to this allele and its application to the cloning of the DNA sequences of other genes in Drosophila is discussed.About:
This article is published in Cell.The article was published on 1981-09-01. It has received 407 citations till now. The article focuses on the topics: Locus (genetics) & DNA sequencing.read more
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A multidrug resistance transporter from human MCF-7 breast cancer cells
L. Austin Doyle,Weidong Yang,Lynne V. Abruzzo,Tammy Krogmann,Y. Gao,Arun K. Rishi,Douglas D. Ross,Douglas D. Ross +7 more
TL;DR: In this article, the authors identify a 2.4-kb mRNA that encodes a 663-aa member of the ATP-binding cassette superfamily of transporters that they termed breast cancer resistance protein (BCRP).
Journal ArticleDOI
Accumulation of Dietary Cholesterol in Sitosterolemia Caused by Mutations in Adjacent ABC Transporters
Knut Erik Berge,Hui Tian,Gregory A. Graf,Liqing Yu,Nick V. Grishin,Joshua Schultz,Peter Kwiterovich,Bei Shan,Robert Barnes,Helen H. Hobbs +9 more
TL;DR: Data suggest that ABCG5 and ABCG8 normally cooperate to limit intestinal absorption and to promote biliary excretion of sterols, and that mutated forms of these transporters predispose to sterol accumulation and atherosclerosis.
Journal ArticleDOI
The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless.
TL;DR: The sequence of the gene suggests that the Dint-1/wingless protein functions in morphogenesis as a signal in cell-cell communication.
Journal ArticleDOI
Targeting induced local lesions IN genomes (TILLING) for plant functional genomics.
TL;DR: The high frequency with which ionizing radiation and certain chemicals can cause genes to mutate made it possible to perform genetic studies and led to the discovery that mutations can be induced.
Journal ArticleDOI
The Molecular Genetics of Cellular Oncogenes
TL;DR: Structural and Functional Properties of Translocation Breakpoints near Cellular Oncogenes and the Functional Significance of Diff erences Between c-onc' s and v-onC' s are studied.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
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Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.