Journal ArticleDOI
Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma
Hina Kalra,Christopher G. Adda,Michael Liem,Ching-Seng Ang,Adam Mechler,Richard J. Simpson,Mark D. Hulett,Suresh Mathivanan +7 more
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TLDR
The OptiPrepTM density gradient method was superior in isolating pure exosomal populations, devoid of highly abundant plasma proteins, and in the context of cellular uptake, the isolated exosomes were able to fuse with target cells revealing that they were indeed biologically active.Abstract:
Exosomes are nanovesicles released by a variety of cells and are detected in body fluids including blood. Recent studies have highlighted the critical application of exosomes as personalized targeted drug delivery vehicles and as reservoirs of disease biomarkers. While these research applications have created significant interest and can be translated into practice, the stability of exosomes needs to be assessed and exosome isolation protocols from blood plasma need to be optimized. To optimize methods to isolate exosomes from blood plasma, we performed a comparative evaluation of three exosome isolation techniques (differential centrifugation coupled with ultracentrifugation, epithelial cell adhesion molecule immunoaffinity pull-down, and OptiPrep(TM) density gradient separation) using normal human plasma. Based on MS, Western blotting and microscopy results, we found that the OptiPrep(TM) density gradient method was superior in isolating pure exosomal populations, devoid of highly abundant plasma proteins. In addition, we assessed the stability of exosomes in plasma over 90 days under various storage conditions. Western blotting analysis using the exosomal marker, TSG101, revealed that exosomes are stable for 90 days. Interestingly, in the context of cellular uptake, the isolated exosomes were able to fuse with target cells revealing that they were indeed biologically active.read more
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Journal ArticleDOI
Establishment of the HeLa Cell Line with Stable Expression of CD63 Exosome Marker Fused with Fluorescent Protein TagRFP and HTBH Tag
TL;DR: This work has generated a stable HeLa cell line expressing exosomal marker CD63 fused with a tagRFP and HTBH tag that constitutes a valuable tool that should allow real-time observations of exosome transport.
Journal ArticleDOI
Extracellular Vesicles in Acute Leukemia: A Mesmerizing Journey With a Focus on Transferred microRNAs.
Mehrdad Izadirad,Zoufang Huang,Farideh Jafari,Amir Ali Hamidieh,Ahmad Gharehbaghian,Yi-Dong Li,Leila Jafari,Zhe-Sheng Chen +7 more
TL;DR: In this paper, a review aimed to briefly shed light on the biology of EVs and to discuss the role of EV-derived miRNAs in the development of acute myeloid leukemia and acute lymphoblastic leukemia.
Journal ArticleDOI
Extracellular vesicle-mediated co-delivery of TRAIL and dinaciclib for targeted therapy of resistant tumors.
Changhong Ke,Huan-rong Hou,Kui Su,Chaohong Huang,Qian Yuan,Shuyi Li,Jianwu Sun,Yue Lin,Chuan-Yu Wu,Yu Zhao,ZhengQiang Yuan +10 more
TL;DR: The data suggest that the co-delivery of TRAIL and Dina by EVs potentially constitutes a novel tumour-targeted therapy, which is highly effective and safe for the treatment of refractory tumors.
Journal ArticleDOI
Size-selective filtration of extracellular vesicles with a movable-layer device.
TL;DR: The device automates size-selective EV filtration that requires laborious multiple washing and separation steps and is envisioned to facilitate molecular analysis and EV-based biomarker discovery that use various biofluids, including blood plasma, urine, and cell culture supernatants.
Dissertation
The proteome of neurofilament-containing heteroaggregates in blood as source of biomarkers for neurodegeneration
TL;DR: The water needs of this region have changed in recent years from being primarily for agricultural purposes to domestic and industrial uses now, and the needs of the energy sector have also changed.
References
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Journal ArticleDOI
Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers
Johan Skog,T. Wurdinger,van Rijn S,Dimphna H. Meijer,Gainche L,Miguel Sena-Esteves,William T. Curry,Bob S. Carter,Anna M. Krichevsky,Xandra O. Breakefield +9 more
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Journal ArticleDOI
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Journal ArticleDOI
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
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