Journal ArticleDOI
Complete mineralization of methylparathion by Pseudomonas sp. A3.
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TLDR
The results from the experiments involving [U-14C]p-nitrophenol provided the evidence for incorporation of carbon into the cellular constituents and release of CO2 from this insecticide.Abstract:
Organophosphorus insecticides are widely used in agriculture. Despite their biodegradable nature, some are highly toxic and their residues are found in the environment. Reports on the mineralization of a spectrum of these insecticides by a single potential strain are scarce. We have isolated a soil isolate, Pseudomonas sp. A3, through enrichment technique, able to degrade methylparathion (MP), malathion, monocrotophos, and Diazinon. The potential of this strain to mineralize MP as a carbon and/or phosphorus source has been evaluated. On hydrolysis of MP, the aromatic portion (p-nitrophenol) was used as a carbon and energy source whereas the alkyl moiety (dithiomethylphosphorothioate) was broken down for the phosphorus source. The results from the experiments involving [U-14C]p-nitrophenol provided the evidence for incorporation of carbon into the cellular constituents and release of CO2 from this insecticide. During the breakdown of MP, nitrite was released as a catabolic by-product.read more
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Biodegradation of the organophosphorus insecticide diazinon by Serratia sp. and Pseudomonas sp. and their use in bioremediation of contaminated soil
TL;DR: The results suggested a strong correlation between microbial activity and chemical processes during diazinon degradation, and isolated bacterial strains may have potential for use in bioremediation of diazInon-contaminated soils.
Journal ArticleDOI
Bioremediation and Phytoremediation of Pesticides: Recent Advances
TL;DR: This review describes the most recent progress in biotechnological approaches for enhancing the capability of microorganisms and plants through the characterization and transfer of pesticide-degrading genes, induction of catabolic pathways, and display of cell surface enzymes.
Journal ArticleDOI
Biodegradation and bioremediation potential of diazinon-degrading Serratia marcescens to remove other organophosphorus pesticides from soils.
TL;DR: Serratia marcescens introduced into sterile soils showed a higher degradation potential (5-13%) for OPPs removal than those observed in non-sterile soil with naturally occurring attenuation, which makes it a suitable strain for bioremediation of soils contaminated with O PPs.
Journal ArticleDOI
Plasmid-borne catabolism of methyl parathion and p-nitrophenol in Pseudomonas sp. strain WBC-3.
TL;DR: The sequence alignment and phylogenetic analysis suggested that MPH and MPD evolved independently from other well-studied organophosphate hydrolases and may be originated from class B beta-lactamase family.
Journal ArticleDOI
Biodegradation of methyl parathion and p-nitrophenol: evidence for the presence of a p-nitrophenol 2-hydroxylase in a Gram-negative Serratia sp. strain DS001.
Suresh B. Pakala,Purushotham Gorla,Aleem Basha Pinjari,Ravi Kumar Krovidi,Rajasekhar Baru,Mahesh Yanamandra,Mike Merrick,Dayananda Siddavattam +7 more
TL;DR: These studies report for the first time the existence of a p-nitrophenol hydroxylase component “A”, typically found in Gram-positive bacteria, in a Gram-negative strain of the genus Serratia.
References
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Journal ArticleDOI
Pathway for Biodegradation of p-Nitrophenol in a Moraxella sp
Jim C. Spain,David T. Gibson +1 more
TL;DR: The results indicate that the pathway for biodegradation of p-nitrophenol involves the initial removal of the nitro group as nitrite and formation of hydroquinone.
Journal ArticleDOI
Parathion hydrolase specified by the Flavobacterium opd gene: relationship between the gene and protein.
W W Mulbry,J S Karns +1 more
TL;DR: The sequence of a 1,693-base-pair plasmid DNA fragment from Flavobacterium sp.
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Plasmid Involvement in Parathion Hydrolysis by Pseudomonas diminuta
TL;DR: An organism identified as Pseudomonas diminuta was found to hydrolyze parathion and hydrolase-negative derivatives were missing a plasmid present in the wild-type organism.
Journal ArticleDOI
Biodegradation of 2,4,5-trichlorophenoxyacetic acid in soil by a pure culture of Pseudomonas cepacia.
TL;DR: A pure culture of Pseudomonas cepacia AC1100 was able to degrade and grow in presence of 2,4,5-trichlorophenoxyacetic acid in soil.
Journal ArticleDOI
Preparation and microbial decomposition of synthetic [14C]ligins.
TL;DR: A definitive assay for microbiological and biochemical research on the biodegradation of lignin was developed using radioactive synthetic lignins specifically labeled in the side chains, aromatic rings or in the methoxyl groups.