Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands
Mitinori Saitou,Mikio Furuse,Hiroyuki Sasaki,Jörg-Dieter Schulzke,Michael Fromm,Hiroshi Takano,Tetsuo Noda,Shoichiro Tsukita +7 more
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TLDR
The generation and analysis of mice carrying a null mutation in the occludin gene suggested that the functions of TJs as well as occlUDin are more complex than previously supposed.Abstract:
Occludin is an integral membrane protein with four transmembrane domains that is exclusively localized at tight junction (TJ) strands. Here, we describe the generation and analysis of mice carrying a null mutation in the occludin gene. Occludin -/- mice were born with no gross phenotype in the expected Mendelian ratios, but they showed significant postnatal growth retardation. Occludin -/- males produced no litters with wild-type females, whereas occludin -/- females produced litters normally when mated with wild-type males but did not suckle them. In occludin -/- mice, TJs themselves did not appear to be affected morphologically, and the barrier function of intestinal epithelium was normal as far as examined electrophysiologically. However, histological abnormalities were found in several tissues, i.e., chronic inflammation and hyperplasia of the gastric epithelium, calcification in the brain, testicular atrophy, loss of cytoplasmic granules in striated duct cells of the salivary gland, and thinning of the compact bone. These phenotypes suggested that the functions of TJs as well as occludin are more complex than previously supposed.read more
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Journal ArticleDOI
Occludin: a novel integral membrane protein localizing at tight junctions.
TL;DR: An integral membrane protein localizing at tight junctions is now identified, which is designated as "occludin," which was revealed by a hydrophilicity plot that was very similar to that of connexin, an integral membraneprotein in gap junctions.
Journal ArticleDOI
Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to Occludin
TL;DR: It is indicated that multiple integral membrane proteins with four putative transmembrane domains, occludin and claudins, constitute TJ strands.
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TL;DR: This chapter reviews the morphological information on intercellular junctions derived from thin-sectioning, negative staining and freeze-cleave techniques, as well as from x-ray diffraction and biochemical investigations, and correlates the structural parameters with known or proposed physiological functions.