Journal ArticleDOI
Computational design of a new hydrogen bond network and at least a 300-fold specificity switch at a protein-protein interface.
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TLDR
This work designed a de novo hydrogen bond network at the DNase-immunity protein interface and confirmed the design with X-ray crystallographic analysis and designed new binding partners that exhibited specificities of at least 300-fold between the cognate and the non-cognate complexes.About:
This article is published in Journal of Molecular Biology.The article was published on 2006-08-04. It has received 154 citations till now. The article focuses on the topics: Molecular recognition & Protein–protein interaction.read more
Citations
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Journal ArticleDOI
The second wave of synthetic biology: from modules to systems
TL;DR: To view cells as true 'programmable' entities, it is now essential to develop effective strategies for assembling devices and modules into intricate, customizable larger scale systems.
Journal ArticleDOI
An improved zinc-finger nuclease architecture for highly specific genome editing
Jeffrey C. Miller,Michael C. Holmes,Jianbin Wang,Dmitry Guschin,Ya-Li Lee,Igor Rupniewski,Christian Beauséjour,Christian Beauséjour,Adam James Waite,Nathaniel Wang,Kenneth Kim,Philip D. Gregory,Carl O. Pabo,Carl O. Pabo,Edward J. Rebar +14 more
TL;DR: Using structure-based design, two variant ZFNs are engineer that modify a native endogenous locus as efficiently as the parental architecture, but with a >40-fold reduction in homodimer function and much lower levels of genome-wide cleavage.
Patent
Method for making antibody fc-heterodimeric molecules using electrostatic steering effects
TL;DR: In this article, the authors proposed methods of making Fc-heterodimeric proteins or polypeptides, including the individual poly-peptide components that comprise the heterodimer.
Journal ArticleDOI
Survey of the year 2003 commercial optical biosensor literature
Rebecca L. Rich,David G. Myszka +1 more
TL;DR: In this overview, 13 papers that should be on everyone's ‘must read’ list for 2003 are spotlighted and examples of how to identify and interpret high‐quality biosensor data are provided.
Journal ArticleDOI
On the binding affinity of macromolecular interactions: daring to ask why proteins interact
TL;DR: It is predicted that any theoretical modelling of these interactions will have to follow an integrative approach considering the biology, chemistry and physics that underlie protein–protein recognition.
References
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Book ChapterDOI
Processing of X-ray diffraction data collected in oscillation mode
Zbyszek Otwinowski,Wladek Minor +1 more
TL;DR: The methods presented in the chapter have been applied to solve a large variety of problems, from inorganic molecules with 5 A unit cell to rotavirus of 700 A diameters crystallized in 700 × 1000 × 1400 A cell.
Journal ArticleDOI
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination
Axel T. Brunger,Axel T. Brunger,Paul D. Adams,G M Clore,W. L. DeLano,Piet Gros,R.W. Grosse-Kunstleve,R.W. Grosse-Kunstleve,Jiansheng Jiang,J. Kuszewski,Michael Nilges,Navraj S. Pannu,Randy J. Read,Luke M. Rice,Thomas Simonson,Gregory L. Warren +15 more
TL;DR: The Crystallography & NMR System (CNS) as mentioned in this paper is a software suite for macromolecular structure determination by X-ray crystallography or solution nuclear magnetic resonance (NMR) spectroscopy.
Journal ArticleDOI
Calculation of protein extinction coefficients from amino acid sequence data
Stanley C. Gill,P H von Hippel +1 more
TL;DR: In this article, a method for calculating accurate molar extinction coefficients for proteins at 280 nm, simply from knowledge of the amino acid composition, was presented, and the method was calibrated against 18 "normal" globular proteins.
Journal ArticleDOI
XtalView/Xfit--A versatile program for manipulating atomic coordinates and electron density.
TL;DR: XFit as mentioned in this paper is a model-building and map viewing program in XView that is used by the structural biology community including researchers in the fields of crystallography, molecular modeling, and electron microscopy.
Journal ArticleDOI
The atomic structure of protein-protein recognition sites.
TL;DR: In this paper, the authors performed an analysis of the recognition sites seen in 75 protein-protein complexes of known three-dimensional structure: 24 protease-inhibitor, 19 antibody-antigen and 32 other complexes, including nine enzymeinhibitors and 11 that are involved in signal transduction.