Orly Dym

TL;DR: The computational design of eight enzymes that use two different catalytic motifs to catalyse the Kemp elimination—a model reaction for proton transfer from carbon—with measured rate enhancements of up to 105 and multiple turnovers are described.

TL;DR: This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.

TL;DR: The production of a recombinant human GCD in a carrot cell suspension culture shows a level of biological activity similar to that of Cerezyme produced in CHO cells, as well as a highly homologous high-resolution three-dimensional structure, determined by X-ray crystallography, indicating the potential safety of prGCD.

TL;DR: A computational strategy is developed and designed an AChE variant bearing 51 mutations that improved core packing, surface polarity, and backbone rigidity and obtained enhanced stability and/or higher yields of soluble and active protein in E. coli.

TL;DR: It is shown that the high-affinity variant RBD-62 can be used as a drug to inhibit infection with SARS-CoV-2 and variants Alpha, Beta and Gamma in vitro and its structure provides a structural basis for future drug and vaccine development and for in silico evaluation of known antibodies.