Journal ArticleDOI
Conditional targeted cell ablation in zebrafish: a new tool for regeneration studies
Silvia Curado,Ryan M. Anderson,Benno Jungblut,Jeff S. Mumm,Eric H. Schroeter,Didier Y.R. Stainier +5 more
TLDR
The findings show that the NTR/Mtz system is effective for temporally and spatially controlled cell ablation in zebrafish, thereby constituting a most promising genetic tool to analyze tissue interactions as well as the mechanisms underlying regeneration.Abstract:
Conditional targeted cell ablation in zebrafish would greatly expand the utility of this genetic model system in developmental and regeneration studies, given its extensive regenerative capabilities. Here, we show that, by combining chemical and genetic tools, one can ablate cells in a temporal- and spatial-specific manner in zebrafish larvae. For this purpose, we used the bacterial Nitroreductase (NTR) enzyme to convert the prodrug Metronidazole (Mtz) into a cytotoxic DNA cross-linking agent. To investigate the efficiency of this system, we targeted three different cell lineages in the heart, pancreas, and liver. Expression of the fusion protein Cyan Fluorescent Protein-NTR (CFP-NTR) under control of tissue-specific promoters allowed us to induce the death of cardiomyocytes, pancreatic beta-cells, and hepatocytes at specific times. Moreover, we have observed that Mtz can be efficiently washed away and that, upon Mtz withdrawal, the profoundly affected tissue can quickly recover. These findings show that the NTR/Mtz system is effective for temporally and spatially controlled cell ablation in zebrafish, thereby constituting a most promising genetic tool to analyze tissue interactions as well as the mechanisms underlying regeneration.read more
Citations
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Journal ArticleDOI
Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation
TL;DR: The data provide the first direct evidence for the source of proliferating cardiomyocytes during zebrafish heart regeneration and indicate that stem or progenitor cells are not significantly involved in this process.
Journal ArticleDOI
Hepatic stellate cells in liver development, regeneration, and cancer
TL;DR: Recent advances in understanding of the formation and characteristics of hepatic stellate cells, as well as their function in liver development, regeneration, and cancer are summarized and evaluated.
Journal ArticleDOI
Comparative Aspects of Animal Regeneration
Jeremy P. Brockes,Anoop Kumar +1 more
TL;DR: The evolutionary origins of regeneration and how regeneration may relate to both agametic reproduction and to ontogeny are considered, including examples of species that are able to regenerate large sections of the body plan.
Journal ArticleDOI
Extensive scar formation and regression during heart regeneration after cryoinjury in zebrafish
TL;DR: The results provide the first evidence that, like mammalian hearts, teleost hearts undergo massive fibrosis after cardiac damage, however, the fish heart can progressively eliminate the scar and regenerate the lost myocardium, indicating that scar formation is compatible with myocardial regeneration and the existence of endogenous mechanisms of scar regression.
Journal ArticleDOI
Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish.
Jon M. Davison,Courtney M. Akitake,Mary G. Goll,Jerry M. Rhee,Nathan J. Gosse,Herwig Baier,Marnie E. Halpern,Steven D. Leach,Michael J. Parsons +8 more
TL;DR: The Tol2-mediated gene/enhancer trapping system together with UAS transgenic lines provides valuable tools for regulated gene expression and for targeted labeling and ablation of specific cell types and tissues during early zebrafish development.
References
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Journal ArticleDOI
Heart Regeneration in Zebrafish
TL;DR: It is demonstrated histologically that zebrafish fully regenerate hearts within 2 months of 20% ventricular resection, showing that injury-induced cardiomyocyte proliferation in zebra fish can overcome scar formation, allowing cardiac muscle regeneration.
Journal ArticleDOI
Conditional control of gene expression in the mouse
TL;DR: Here, I review binary systems used to analyse complex and multi-staged biological processes, such as embryogenesis and cancer, with unprecedented precision and discuss certain studies that exemplify the advantages and limitations of each system.
Journal ArticleDOI
A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish.
Koichi Kawakami,Hisashi Takeda,Noriko Kawakami,Makoto Kobayashi,Naoto Matsuda,Masayoshi Mishina +5 more
TL;DR: A transposon-based gene trap vector containing a splice acceptor and the GFP gene was constructed, performed a pilot screen for gene trapping, and obtained fish expressing GFP in temporally and spatially restricted patterns.
Journal ArticleDOI
Germ-line transmission of a myocardium-specific GFP transgene reveals critical regulatory elements in the cardiac myosin light chain 2 promoter of zebrafish.
TL;DR: Interestingly, a germ‐line of zebrafish whose GFP appeared ectopically in all muscle types (heart, skeletal, and smooth) was generated by injecting the fragment including a single nucleotide mutation from G to A at −119, evidence that A at−119 combined with neighboring nucleotides to create a consensus sequence for binding myocyte‐specific enhancer factor‐2.