Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Delta(9)-desaturase.
J. M. Griinari,Benjamin A. Corl,Shannon H. Lacy,P. Y. Chouinard,K. V. V. Nurmela,Dale E. Bauman +5 more
TLDR
Results demonstrate that endogenous synthesis of CLA from trans-11 18:1 represented the primary source of CLA in milk fat of lactating cows.Abstract:
Conjugated linoleic acid (CLA) is a naturally occurring anticarcinogen found in milk fat and body fat of ruminants. Although CLA is an intermediate in ruminal biohydrogenation of linoleic acid, we hypothesized that its primary source was from endogenous synthesis. This would involve Delta(9)-desaturase and synthesis from trans-11 18:1, another intermediate in ruminal biohydrogenation. Our first experiment supplied lactating cows (n = 3) with trans-11 18:1 by abomasal infusion and examined the potential for endogenous synthesis by measuring changes in milk fat CLA. By d 3, infusion of trans-11 18:1 resulted in a 31% increase in concentration of cis-9, trans-11 CLA in milk fat, demonstrating that an active pathway for endogenous synthesis of CLA exists. Our second experiment examined the quantitative importance of endogenous synthesis of CLA in lactating cows (n = 3) by abomasally infusing a putative stimulator (retinol palmitate) or an inhibitor (sterculic oil) of Delta(9)-desaturase. Infusion of retinol palmitate had no influence on milk fatty acid desaturation, and yield of CLA in milk fat was not altered. However, sterculic oil infusion decreased the concentration of CLA in milk fat by 45%. Consistent with Delta(9)-desaturase inhibition, the sterculic oil treatment also altered the milk fat concentration of other Delta(9)-desaturase products as indicated by the two- to threefold increase in the ratios of 14:0 to 14:1(,) 16:0 to 16:1 and 18:0 to cis-18:1. Using changes in the ratio of 14:0 to 14:1 as an indication of the extent of Delta(9)-desaturase inhibition with the sterculic oil treatment, an estimated 64% of the CLA in milk fat was of endogenous origin. Overall, results demonstrate that endogenous synthesis of CLA from trans-11 18:1 represented the primary source of CLA in milk fat of lactating cows.read more
Citations
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Stearoyl CoA desaturase (SCD) gene polymorphisms in Italian cattle breeds
TL;DR: Investigating the polymorphism of three previously reported single nucleotide polymorphisms located in exon 5 of the SCD gene in 11 cattle breeds raised in Italy and selected for different production goals evidenced a high variability in the allele frequencies across breeds and detected three novel haplotypes.
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Chemical composition of water buffalo milk and its low-fat symbiotic yogurt development
TL;DR: In this paper, the symbiotic low-fat buffalo milk yogurt was evaluated for physicochemical and microbiological properties and for the survivability of probiotics during ten weeks of storage.
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Fresh forage and solin supplementation on conjugated linoleic acid levels in plasma and milk.
TL;DR: Solin supplementation in a lactation diet is a superior method to increase CLA levels in milk fat than feeding fresh forage alone.
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Dietary supplementation with dried olive pomace in dairy cows modifies the composition of fatty acids and the aromatic profile in milk and related cheese
Federica Castellani,Andrea Vitali,Nadia Bernardi,Elettra Marone,Fiorentina Palazzo,Lisa Grotta,Giuseppe Martino +6 more
TL;DR: It is pointed out that DOP supplementation may improve the nutritional and nutraceutical properties and modify the aroma of milk and derived cheese.
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Effect of oil supplementation of a diet containing a high concentration of starch on levels of trans fatty acids and conjugated linoleic acids in bovine milk
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