Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Delta(9)-desaturase.
J. M. Griinari,Benjamin A. Corl,Shannon H. Lacy,P. Y. Chouinard,K. V. V. Nurmela,Dale E. Bauman +5 more
TLDR
Results demonstrate that endogenous synthesis of CLA from trans-11 18:1 represented the primary source of CLA in milk fat of lactating cows.Abstract:
Conjugated linoleic acid (CLA) is a naturally occurring anticarcinogen found in milk fat and body fat of ruminants. Although CLA is an intermediate in ruminal biohydrogenation of linoleic acid, we hypothesized that its primary source was from endogenous synthesis. This would involve Delta(9)-desaturase and synthesis from trans-11 18:1, another intermediate in ruminal biohydrogenation. Our first experiment supplied lactating cows (n = 3) with trans-11 18:1 by abomasal infusion and examined the potential for endogenous synthesis by measuring changes in milk fat CLA. By d 3, infusion of trans-11 18:1 resulted in a 31% increase in concentration of cis-9, trans-11 CLA in milk fat, demonstrating that an active pathway for endogenous synthesis of CLA exists. Our second experiment examined the quantitative importance of endogenous synthesis of CLA in lactating cows (n = 3) by abomasally infusing a putative stimulator (retinol palmitate) or an inhibitor (sterculic oil) of Delta(9)-desaturase. Infusion of retinol palmitate had no influence on milk fatty acid desaturation, and yield of CLA in milk fat was not altered. However, sterculic oil infusion decreased the concentration of CLA in milk fat by 45%. Consistent with Delta(9)-desaturase inhibition, the sterculic oil treatment also altered the milk fat concentration of other Delta(9)-desaturase products as indicated by the two- to threefold increase in the ratios of 14:0 to 14:1(,) 16:0 to 16:1 and 18:0 to cis-18:1. Using changes in the ratio of 14:0 to 14:1 as an indication of the extent of Delta(9)-desaturase inhibition with the sterculic oil treatment, an estimated 64% of the CLA in milk fat was of endogenous origin. Overall, results demonstrate that endogenous synthesis of CLA from trans-11 18:1 represented the primary source of CLA in milk fat of lactating cows.read more
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Differential action of trans-10, cis-12 conjugated linoleic acid on adipocyte differentiation of ovine and 3T3-L1 preadipocytes.
Tomoyo Iga,Takumi Satoh,S. Yamamoto,K. Fukui,S. H. Song,K. C. Choi,Sang Gun Roh,Shinichi Sasaki +7 more
TL;DR: The results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadendipocytes.
Journal ArticleDOI
The effects of diets containing two corn stubble levels and three non-hydrogenated lipids sources on fattening performance, carcase, and meat quality of male hair-lambs
J. Efrén Ramírez-Bribiesca,Laura Hernández-Cruz,Rosy G. Cruz-Monterrosa,Isabel Guerrero-Legarreta,Daniel Mota-Rojas,Adolfo Armando Rayas-Amor,Ignacio A. Domínguez-Vara,Leonel Avendaño-Reyes,Luis Corona-Gochi,Mario Cuchillo-Hilario +9 more
TL;DR: Protein content and juiciness in the meat improve with NDF-29 (as corn stubble) on a diet, and Safflower and linseed oils included in the diet at 3% improve the content of conjugated linoleic acid in lamb meat.
Journal ArticleDOI
Identification of feeding pattern and their impact on milk fatty acid profiles from traditional dairy cows in Pangalengan Sub-district
TL;DR: In this paper , the authors identify the relationship between the feeding system and milk FA profile in the Pangalengan sub-district using two-step post observatory research.
Journal ArticleDOI
Current Knowledge on Source and Synthesis of Conjugated Linoleic Acid (CLA): A Review
TL;DR: Fatty acids can be categorized according to their number of double bonds and PUFA having 20 or more carbon atoms are usually termed as LCPUFA both are essential for physiological activities of the organism.
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TL;DR: In this article, an improved method for quantifying linoleic acid (CLA) in food was developed, which was used to produce a data base of more than 90 food items including meat, poultry, seafood, dairy products, plant oils, and infant and processed foods.
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