Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Delta(9)-desaturase.
J. M. Griinari,Benjamin A. Corl,Shannon H. Lacy,P. Y. Chouinard,K. V. V. Nurmela,Dale E. Bauman +5 more
TLDR
Results demonstrate that endogenous synthesis of CLA from trans-11 18:1 represented the primary source of CLA in milk fat of lactating cows.Abstract:
Conjugated linoleic acid (CLA) is a naturally occurring anticarcinogen found in milk fat and body fat of ruminants. Although CLA is an intermediate in ruminal biohydrogenation of linoleic acid, we hypothesized that its primary source was from endogenous synthesis. This would involve Delta(9)-desaturase and synthesis from trans-11 18:1, another intermediate in ruminal biohydrogenation. Our first experiment supplied lactating cows (n = 3) with trans-11 18:1 by abomasal infusion and examined the potential for endogenous synthesis by measuring changes in milk fat CLA. By d 3, infusion of trans-11 18:1 resulted in a 31% increase in concentration of cis-9, trans-11 CLA in milk fat, demonstrating that an active pathway for endogenous synthesis of CLA exists. Our second experiment examined the quantitative importance of endogenous synthesis of CLA in lactating cows (n = 3) by abomasally infusing a putative stimulator (retinol palmitate) or an inhibitor (sterculic oil) of Delta(9)-desaturase. Infusion of retinol palmitate had no influence on milk fatty acid desaturation, and yield of CLA in milk fat was not altered. However, sterculic oil infusion decreased the concentration of CLA in milk fat by 45%. Consistent with Delta(9)-desaturase inhibition, the sterculic oil treatment also altered the milk fat concentration of other Delta(9)-desaturase products as indicated by the two- to threefold increase in the ratios of 14:0 to 14:1(,) 16:0 to 16:1 and 18:0 to cis-18:1. Using changes in the ratio of 14:0 to 14:1 as an indication of the extent of Delta(9)-desaturase inhibition with the sterculic oil treatment, an estimated 64% of the CLA in milk fat was of endogenous origin. Overall, results demonstrate that endogenous synthesis of CLA from trans-11 18:1 represented the primary source of CLA in milk fat of lactating cows.read more
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Changes in Rumen Microbial Profiles and Subcutaneous Fat Composition When Feeding Extruded Flaxseed Mixed With or Before Hay.
TL;DR: A number of pathways for ALA biohydrogenation are operating concurrently in the rumen, with their balance being influenced by diet and driven by less abundant species rather than members of the core bacterial population.
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Milk Fat Depression: Etiology, Theories, and Soluble Carbohydrate Interactions
L. E. Koch,G.J. Lascano +1 more
TL;DR: A review of the knowledge available on milk fat depression, microbial influences, and how soluble carbohydrate fractions influence on biohydrogenation can be found in this paper, with a focus on how different nutrients interact in the rumen and affect the production of these fat inhibiting isomers.
Journal ArticleDOI
Supplementation with Cashew Nut and Cottonseed Meal to Modify Fatty Acid Content in Lamb Meat.
Elzania Sales Pereira,Ivone Yurika Mizubuti,Ronaldo Lopes Oliveira,Andréa Pereira Pinto,Edson Luis de Azambuja Ribeiro,Carla Renata Figueiredo Gadelha,A. C. N. Campos,Marília Williani Filgueira Pereira,Maria Socorro de Souza Carneiro,Paulo César Lopes de Arruda,Luciano Pinheiro da Silva +10 more
TL;DR: The use of cashew nuts in the diet resulted in an increase in the C18:2c9t11 content of the lamb meat, which improved the nutritional characteristics of the fat.
Journal ArticleDOI
Crude glycerin in supplement to primiparous lactating cows grazing on tropical pasture: nutritional and productive characteristics
Hermógenes Almeida de Santana Júnior,Mauro Pereira de Figueiredo,Elizangela Oliveira Cardoso,Fabrício Bacelar Lima Mendes,George Abreu Filho,Alyson Andrade Pinheiro,Pablo Teixeira Viana,João Paulo Santos Roseira +7 more
TL;DR: For primiparous lactating cows grazing on tropical pasture, up to 389 g of crude glycerin/kg of dietary supplement can be included without nutritional and productive interference.
Journal ArticleDOI
9cis,11trans conjugated linoleic acid (CLA) is synthesised and desaturated into conjugated 18:3 in bovine adipose tissues
TL;DR: Bovine adipose tissues of the growing ruminant were especially involved in the endogenous synthesis of CLA from VA and in its desaturation into conjugated derivative, mainly 6 cis, 9cis,11trans 18:3, of which biological properties need to be elucidated.
References
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