Coordinated rearrangements between cytoplasmic and periplasmic domains of the membrane protein complex ExbB-ExbD of Escherichia coli.
Aleksandr Sverzhinsky,Lucien Fabre,Andrew L. Cottreau,Damien M.P. Biot-Pelletier,Sofia Khalil,Mihnea Bostina,Isabelle Rouiller,James W. Coulton +7 more
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Copurified ExbB with ExbD as an ∼240 kDa protein-detergent complex, measured by light scattering and by native gels to reconstruct the particles in three structural states followed by sorting of the single particles and refinement of each state.About:
This article is published in Structure.The article was published on 2014-05-06 and is currently open access. It has received 18 citations till now. The article focuses on the topics: Periplasmic space & Membrane protein complex.read more
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Structural insight into the role of the Ton complex in energy transduction
Herve Celia,Nicholas Noinaj,Stanislav D. Zakharov,Enrica Bordignon,Istvan Botos,Monica Santamaria,Travis J. Barnard,William A. Cramer,Roland Lloubès,Susan K. Buchanan +9 more
TL;DR: Electrophysiology studies show that the Ton subcomplex forms pH-sensitive cation-selective channels and provide insight into the mechanism by which it may harness the proton motive force to produce energy.
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The Role of Iron and Siderophores in Infection, and the Development of Siderophore Antibiotics
TL;DR: The mechanisms implicated in iron uptake and the challenges associated with the design and utilization of siderophore-mimicking antibiotics are discussed.
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Hexameric and pentameric complexes of the ExbBD energizer in the Ton system
Saori Maki-Yonekura,Rei Matsuoka,Yoshiki Yamashita,Hirofumi Shimizu,Maiko Tanaka,Fumie Iwabuki,Koji Yonekura +6 more
TL;DR: This work presents structures of hexameric complexes of ExbB and ExbD revealed by X-ray crystallography and single particle cryo-EM and proposes models for activation/inactivation associated with hexamer and pentamer formation and utilization of proton motive force.
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ROSET Model of TonB Action in Gram-negative Bacterial Iron Acquisition
TL;DR: The ROSET model postulates a mechanism for the transfer of energy from the IM to the OM, triggering iron uptake, and the equilibration of the TonB C terminus between the dimeric and monomeric forms that engage in different binding reactions allows the identification of iron-loaded LGP and then the internalization of iron through their trans-outer membrane β-barrels.
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Microbial siderophore-based iron assimilation and therapeutic applications
TL;DR: The study of biosynthetic pathways to natural siderophores augments the understanding of the complex mechanisms of bacterial iron acquisition, and enables a complimentary approach to address virulence through the interruption of sidersophore biosynthesis or utilization by targeting the key enzymes to the siderophile pathways.
References
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Molecular Basis of Bacterial Outer Membrane Permeability Revisited
TL;DR: This review summarizes the development in the field since the previous review and begins to understand how this bilayer of the outer membrane can retard the entry of lipophilic compounds, owing to increasing knowledge about the chemistry of lipopolysaccharide from diverse organisms and the way in which lipopoly Saccharide structure is modified by environmental conditions.
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Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy.
TL;DR: The hand determination and refinement optimization procedure is applied to image pairs of the dihydrolipoyl acetyltransferase (E2) catalytic core of the pyruvate dehydrogenase complex from Bacillus stearothermophilus taken by low-dose electron cryomicroscopy.
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TonB-Dependent Transporters: Regulation, Structure, and Function
TL;DR: Recent progress in understanding regulation, structure, and function in TBDTs is summarized and questions remaining to be answered are summarized.
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Volume changes on protein folding.
TL;DR: The exceptionally high density of the protein interior shown here implies that packing forces play a more important role in protein stability than has been believed hitherto.
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Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization.
Sjors H.W. Scheres,Haixiao Gao,Mikel Valle,Gabor T. Herman,Paul P. B. Eggermont,Joachim Frank,José María Carazo +6 more
TL;DR: This work presents a maximum likelihood–based classification method that does not depend on prior knowledge about the structural variability, and demonstrates its effectiveness for two macromolecular assemblies with different types of conformational variability: the Escherichia coli ribosome and Simian virus 40 large T-antigen.
Related Papers (5)
Quantification of known components of the Escherichia coli TonB energy transduction system: TonB, ExbB, ExbD and FepA
TonB or not TonB: is that the question?This paper is one of a selection of papers published in a Special Issue entitled CSBMCB 53rd Annual Meeting — Membrane Proteins in Health and Disease, and has undergone the Journal’s usual peer review process.
Karla D. Krewulak,Hans J. Vogel +1 more