Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours
David Glenn,Huidan Zhang,Narayanan Kasthuri,Richard Schalek,Pik Kwan Lo,Alexei Trifonov,Hongkun Park,Jeff W. Lichtman,Ronald L. Walsworth +8 more
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TLDR
The results pave the way for the use of such nanoparticles for targeted labeling of surfaces to provide nanoscale mapping of molecular composition, indicated by cathodoluminescence colour, simultaneously acquired with structural electron images in a single instrument.Abstract:
Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images, and rapid optical signal degradation under electron beam irradiation. Here, we introduce a new approach to solve these problems: imaging of stable optical cathodoluminescence emitted in a scanning electron microscope by nanoparticles with controllable surface chemistry. We demonstrate well-correlated cathodoluminescence and secondary electron images using three species of semiconductor nanoparticles that contain defects providing stable, spectrally-distinguishable cathodoluminescence. We also demonstrate reliable surface functionalization of the particles. The results pave the way for the use of such nanoparticles for targeted labeling of surfaces to provide nanoscale mapping of molecular composition, indicated by cathodoluminescence colour, simultaneously acquired with structural electron images in a single instrument.read more
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Mass production and dynamic imaging of fluorescent nanodiamonds
Yi Ren Chang,Hsu-Yang Lee,Kowa Chen,Chun-Chieh Chang,Dung-Sheng Tsai,Chi Cheng Fu,Tsong-Shin Lim,Yan-Kai Tzeng,Chia Yi Fang,Chau-Chung Han,Huan-Cheng Chang,Wunshain Fann +11 more
TL;DR: It is shown that bright fluorescent nanodiamonds can be produced in large quantities by irradiating synthetic diamond nanocrystallites with helium ions, and the fluorescence is sufficiently bright and stable to allow three-dimensional tracking of a single particle within the cell by means of either one- or two-photon-excited fluorescence microscopy.
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Correlated light and electron microscopy: ultrastructure lights up!
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Scanning Electron Microscopy: Physics of Image Formation and Microanalysis
TL;DR: In this article, the authors describe the physics of a scanning electron microscope, including: Electron Optics of a Scanning Electron Microscope., Electron Scattering and Diffusion, Emission of Backscattered and Secondary Electrons, Electron Detectors and Spectrometers, Image Contrast and Signal Processing, and Electron-Beam Induced Current and Cathodoluminescence.
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Network anatomy and in vivo physiology of visual cortical neurons
Davi D. Bock,Wei-Chung Allen Lee,Aaron M. Kerlin,Mark L. Andermann,Greg Hood,Arthur W. Wetzel,Sergey Yurgenson,Edward R. Soucy,Hyon Suk Kim,R. Clay Reid +9 more
TL;DR: This work used two-photon calcium imaging to characterize a functional property—the preferred stimulus orientation—of a group of neurons in the mouse primary visual cortex and large-scale electron microscopy of serial thin sections was used to trace a portion of these neurons’ local network.