Cryo-EM reveals mechanistic insights into lipid-facilitated polyamine export by human ATP13A2.
Atsuhiro Tomita,Takashi Daiho,Tsukasa Kusakizako,Keitaro Yamashita,Satoshi Ogasawara,Takeshi Murata,Tomohiro Nishizawa,Tomohiro Nishizawa,Osamu Nureki +8 more
TLDR
In this article, the structure of human ATP13A2 was analyzed under four different conditions, revealing the structural coupling between the polyamine binding and the dephosphorylation.About:
This article is published in Molecular Cell.The article was published on 2021-11-18 and is currently open access. It has received 15 citations till now. The article focuses on the topics: Polyamine binding & Polyamine.read more
Citations
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Autoinhibition and regulation by phosphoinositides of ATP8B1, a human lipid flippase associated with intrahepatic cholestatic disorders
TL;DR: In this paper , the authors reported functional studies and the cryo-EM structure of the human lipid flippase ATP8B1-CDC50A at 3.1 Å resolution.
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P5B-ATPases in the mammalian polyamine transport system and their role in disease.
Mujahid Azfar,Sarah van Veen,Marine Houdou,Norin Nabil Hamouda,Jan Eggermont,Peter Vangheluwe +5 more
TL;DR: The role of P5B-ATPases ATP13A2-5 transporters in the endosomal system has been investigated in this paper , where the authors discuss recent breakthroughs on their biochemical and structural properties as well as their implications for disease and therapy.
Journal ArticleDOI
Cryo-EM of the ATP11C flippase reconstituted in Nanodiscs shows a distended phospholipid bilayer inner membrane around transmembrane helix 2
TL;DR: In this paper , the structure and function of ATP11C in a natural lipid environment were characterized using single-particle cryo-electron microscopy (cryo-EM) and they showed that the membrane boundary varies upon conformational changes of the enzyme and is no longer flat around the protein.
Journal ArticleDOI
Structure and transport mechanism of the human calcium pump SPCA1
TL;DR: In this article , the authors determined six cryo-electron microscopy (cryo-EM) structures of human SPCA1 (hSPCA1) in a series of intermediate states, revealing a near-complete conformational cycle.
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Novel green fluorescent polyamines to analyze ATP13A2 and ATP13A3 activity in the mammalian polyamine transport system
TL;DR: In this article , the authors showed that fluorescently labeled polyamines are genuine substrates of ATP13A2 and showed that they can be used to measure polyamine uptake in ATP13a2 and ATP13-A3-dependent cell models resembling radiolabeled polyamines uptake.
References
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James C. Phillips,Rosemary Braun,Wei Wang,James C. Gumbart,Emad Tajkhorshid,Elizabeth Villa,Christophe Chipot,Robert D. Skeel,Laxmikant V. Kale,Klaus Schulten +9 more
TL;DR: NAMD as discussed by the authors is a parallel molecular dynamics code designed for high-performance simulation of large biomolecular systems that scales to hundreds of processors on high-end parallel platforms, as well as tens of processors in low-cost commodity clusters, and also runs on individual desktop and laptop computers.
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Comparative Protein Modelling by Satisfaction of Spatial Restraints
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