Journal ArticleDOI
Detection and measurement of nucleotide excision repair synthesis by mammalian cell extracts in vitro
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TLDR
Methods for a versatile repair synthesis assay are described and Protocols are given for preparing plasmid DNA substrates, preparing whole cell extracts, performing repair synthesis reactions, and analyzing the data obtained.About:
This article is published in Methods.The article was published on 1995-04-01. It has received 81 citations till now. The article focuses on the topics: Nucleotide excision repair & DNA.read more
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Second pathway for completion of human DNA base excision‐repair: reconstitution with purified proteins and requirement for DNase IV (FEN1)
Arne Klungland,Tomas Lindahl +1 more
TL;DR: The structure‐specific nuclease DNase IV (FEN1) was essential for repair of a reduced AP site, which occurred through the long‐patch BER pathway, which was largely dependent on DNA polymerase β in cell extracts, but the reaction could be reconstituted with either DNA polymerases β or δ.
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MGMT: its role in cancer aetiology and cancer therapeutics.
TL;DR: The DNA-repair protein O6-alkylguanine DNA alkyltransferase (AGT) has a wide range of activity in normal tissues and its evolutionary conservation indicates a fundamental role in cell physiology and maintenance of the genome.
Journal ArticleDOI
ROS1, a repressor of transcriptional gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase
Zhizhong Gong,Teresa Morales-Ruiz,Rafael R. Ariza,Teresa Roldán-Arjona,Lisa K. David,Jian-Kang Zhu +5 more
TL;DR: It is suggested that ROS1 is a DNA repair protein that represses homology-dependent transcriptional gene silencing by demethylating the target promoter DNA.
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The mitochondrial energy transduction system and the aging process.
TL;DR: Dysfunctional mitochondria in aged rodents are characterized, besides decreased electron transfer and O(2) uptake, by an increased content of oxidation products of phospholipids, proteins and DNA, a decreased membrane potential, and increased size and fragility.