Journal ArticleDOI
Direct binding of protein to magnetic particles
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TLDR
A new method of binding bovine serum albumin on to freshly precipitated magnetic particles is reported, and may prove to be applicable to radio-immuno assays (binding of antibodies to magnetic particles), cell and enzyme immobilisation and in affinity chromatography.Abstract:
A new method of binding bovine serum albumin on to freshly precipitated magnetic particles is reported. The binding was confirmed by electron micrograph studies, magnetic measurements and FTIR spectroscopy. Under optimum conditions more than 90% of the protein was bound to the magnetic particles. When alkaline phosphatase was immo-bilised using this method, it retained 75% enzyme activity. The method may prove to be applicable to radio-immuno assays (binding of antibodies to magnetic particles), cell and enzyme immobilisation and in affinity chromatographyread more
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TOPICAL REVIEW: Applications of magnetic nanoparticles in biomedicine
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Applications of magnetic nanoparticles in biomedicine
TL;DR: The physical principles underlying some current biomedical applications of magnetic nanoparticles are reviewed and the relevant physics of magnetic materials and their responses to applied magnetic fields are surveyed.
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Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications
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TL;DR: This review describes recent advances in the synthesis of biomolecule-nanoparticle/nanorod hybrid systems and the application of such assemblies in the generation of 2D and 3D ordered structures in solutions and on surfaces.
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Magnetic nanoparticles: design and characterization, toxicity and biocompatibility, pharmaceutical and biomedical applications.
TL;DR: Biocompatibility, Pharmaceutical and Biomedical Applications L. Harivardhan Reddy,‡ Jose ́ L. Arias, Julien Nicolas,† and Patrick Couvreur*,†.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Ionic ferrofluids: A crossing of chemistry and physics
TL;DR: In this article, a phase diagram is built up as a function of ionic strength and preliminary results on the magnetic-field-induced phase separation are given, where Colloidal stability is considered with respect to various particle interaction parameters.
Journal ArticleDOI
Magnetic fluid as a possible drug carrier for thrombosis treatment
A.N. Rusetski,Enno K. Ruuge +1 more
TL;DR: In this article, the possibility of using a composition of ferrofluid with fibrinolytic enzyme for thrombosis treatment has been demonstrated and the main factors that affect the magnetic capture in the occluded branch of the blood vessel of such a composition and the factors that affecting magnetically holding the ferro-fluid drop on the tube wall in the stream of a medium were obtained.
Journal ArticleDOI
Chemical modification of purple membranes: role of arginine and carboxylic acid residues in bacteriorhodopsin
Lester Packer,Lester Packer,Lester Packer,Stephanie Tristram,Stephanie Tristram,Stephanie Tristram,Jeffrey M. Herz,Jeffrey M. Herz,Jeffrey M. Herz,Charlotte Russell,Charlotte Russell,Charlotte Russell,C.L. Borders,C.L. Borders,C.L. Borders +14 more
TL;DR: The results suggest that deprotonation and reprotonation of the retinal Schiff base are essential for proton translocation across the membranes of Halobacterium halobium.
Journal ArticleDOI
Selective HGMS of colloidal magnetite-binding cells from whole blood
TL;DR: Selective HGMS with a high enrichment factor for lymphocytes from whole blood is achieved and magnetophoretic study provides information on the adsorption peculiarities of colloidal magnetite on various blood cells.
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