Journal ArticleDOI
Direct induction of dictyostelium prestalk gene expression by DIF provides evidence that DIF is a morphogen
Jeffrey G. Williams,Adriano Ceccarelli,S.J. McRobbie,Hiro Mahbubani,Robert R. Kay,A. Early,M. Berks,K A Jermyn +7 more
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The demonstration that DIF induces a gene normally only expressed in the prestalk zone of the slug provides strong evidence that Dif is a Dictyostelium morphogen.About:
This article is published in Cell.The article was published on 1987-04-24. It has received 202 citations till now. The article focuses on the topics: Cellular differentiation & Morphogen.read more
Citations
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Journal ArticleDOI
A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats.
TL;DR: It is shown here that all three antibodies react with a synthetic peptide with an amino acid sequence corresponding to that predicted by the tandem repeat, allowing a directed approach to the development of tumor-specific antibodies using synthetic peptides as immunogens.
Book ChapterDOI
Neurotransmitters as morphogens.
TL;DR: This chapter presents considerable evidence for morphogenetic functions of neurotransmitters during embryonic development, a role that may derive from their appearance early in evolution, still evidenced today by their presence in primitive organisms.
Journal ArticleDOI
SH2 Signaling in a Lower Eukaryote: A STAT Protein That Regulates Stalk Cell Differentiation in Dictyostelium
Takefumi Kawata,Andrej Shevchenko,Masashi Fukuzawa,Keith A Jermyn,Nicholas F. Totty,Natasha V. Zhukovskaya,Alistair E. Sterling,Matthias Mann,Jeffrey G. Williams +8 more
TL;DR: It is shown that the TTGA-binding factor is a STAT protein, which functions via the reciprocal interaction of a phosphotyrosine residue on one molecule with an SH2 domain on a dimerizing partner, and will bind specifically to a mammalian interferon-stimulated response element.
Journal ArticleDOI
Glycogen synthase kinase 3 regulates cell fate in Dictyostelium.
TL;DR: It is proposed that cAMP acts through a common pathway that requires GSK-3 and determines the proportion of prespore and pstB cells and is cloned gskA, which encodes the Dictyostelium homolog of glycogen synthase kinase 3 (GSK-3), and discovered that it is required for both cAMP effects.
Journal ArticleDOI
Insights into morphogenesis from a simple developmental system.
TL;DR: Genetic and genomic studies, combined with real-time imaging, have identified key pathways that regulate morphogenesis in D. discoideum, and which are likely to have similar roles in metazoans.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
The Chemical Basis of Morphogenesis
TL;DR: In this article, it is suggested that a system of chemical substances, called morphogens, reacting together and diffusing through a tissue, is adequate to account for the main phenomena of morphogenesis.
Journal ArticleDOI
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Jeffrey Vieira,Joachim Messing +1 more
TL;DR: A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed and a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM).
Journal ArticleDOI
A theory of biological pattern formation.
Alfred Gierer,Hans Meinhardt +1 more
TL;DR: It is shown that relatively simple molecular mechanisms based on auto- and cross catalysis can account for a primary pattern of morphogens to determine pattern formation of the tissue, and the theory is applied to quantitative data on hydra and is shown to account for activation and inhibition of secondary head formation.