Downregulation of miR-7 upregulates Cullin 5 (CUL5) to facilitate G1/S transition in human hepatocellular carcinoma cells.
TLDR
It is demonstrated that miR‐7 was downregulated in hepatocellular carcinoma (HCC) tissues compared to adjacent non‐tumor tissue, and it may function as a tumor suppressor in HCC.Abstract:
MicroRNAs (miRNAs) are small, non-coding RNAs that participate in the regulation of gene expression. In this study, we demonstrate that miR-7 was downregulated in hepatocellular carcinoma (HCC) tissues compared to adjacent non-tumor tissue. Over-expression of miR-7 in QGY-7703 and HepG2 cell lines inhibited colony formation and induced G1/S phase arrest, whereas knockdown of miR-7 produced the opposite phenotype. A tumor suppressor gene, CUL5, was identified as a direct target of miR-7, and CUL-5 is upregulated upon the binding of miR-7 to its 3'UTR. Furthermore, suppression of CUL5 also suppressed cell colony formation and induced cell cycle arrest. Ectopic expression of CUL5 abrogated the effects of miR-7 inhibition on QGY-7703 and HepG2 cell lines. These results indicate that miR-7 suppresses colony formation and causes cell cycle arrest via upregulation of CUL5, and it may function as a tumor suppressor in HCC.read more
Citations
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Circular RNA circMTO1 acts as the sponge of microRNA-9 to suppress hepatocellular carcinoma progression.
Dan Han,Jiangxue Li,Huamin Wang,Xiaoping Su,Jin Hou,Yan Gu,Cheng Qian,Yun Lin,Xiang Liu,Mingyan Huang,Nan Li,Weiping Zhou,Yizhi Yu,Xuetao Cao +13 more
TL;DR: In this article, the expression profile and function of circRNAs in human hepatocellular carcinoma (HCC) remain to be investigated, and the authors used a biotin-labeled circMTO1 probe to perform RNA in vivo precipitation in HCC cells.
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Circular RNA participates in the carcinogenesis and the malignant behavior of cancer
Zhen-Jun Zhao,Jun Shen +1 more
TL;DR: CircRNA, together with its gene silencing ability, also shows its potential in RNA interference (RNAi) therapy by binding to target RNAs, which provides a novel perspective in cancer treatment.
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The Circular RNA Cdr1as Act as an Oncogene in Hepatocellular Carcinoma through Targeting miR-7 Expression.
TL;DR: It is demonstrated that Cdr1as expression was upregulated in HCC tissues compared with the adjacent non-tumor tissues and knockdown of Cdr 1as suppressed the HCC cell proliferation and invasion through targeting miR-7.
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The circular RNA ciRS-7 (Cdr1as) acts as a risk factor of hepatic microvascular invasion in hepatocellular carcinoma.
TL;DR: Up-regulated ciRS-7 expression was not only an independent risk factor of hepatic MVI but also had a capable predictive ability for MVI and a novel therapy target for restraining MVI, as suggested by data suggested.
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The emerging landscape of circular RNA ciRS-7 in cancer (Review)
TL;DR: A recently identified circRNA, ciRS-7, can regulate the activities of miRNs, mRNAs, and RBP to exert specific biological effects.
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