Journal ArticleDOI
Effect of nitric oxide production on the redox modulatory site of the NMDA receptor-channel complex
Sizheng Z. Lei,Zhuo Hua Pan,Sanjay K. Aggarwal,Huei Sheng Vincent Chen,Jonathan Hartman,Nikolaus J. Sucher,Stuart A. Lipton,Stuart A. Lipton +7 more
TLDR
The results demonstrate a new pathway for NO regulation of physiological function that is not via cGMP, but instead involves reactions with membrane-bound thiol groups on the NMDA receptor-channel complex.About:
This article is published in Neuron.The article was published on 1992-06-01. It has received 762 citations till now. The article focuses on the topics: NMDA receptor & Nitric oxide.read more
Citations
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Nitric Oxide and Peroxynitrite in Health and Disease
TL;DR: Current evidence indicates that most of the cytotoxicity attributed to NO is rather due to peroxynitrite, produced from the diffusion-controlled reaction between NO and another free radical, the superoxide anion, which is presented in detail in this review.
Journal ArticleDOI
Biochemistry of nitric oxide and its redox-activated forms
TL;DR: The integration of this chemistry with current perspectives of NO biology illuminates many aspects of NO biochemistry, including the enzymatic mechanism of synthesis, the mode of transport and targeting in biological systems, the means by which its toxicity is mitigated, and the function-regulating interaction with target proteins.
Journal ArticleDOI
A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds
Stuart A. Lipton,Yun-Beom Choi,Yun-Beom Choi,Zhuo Hua Pan,Zhuo Hua Pan,Sizheng Z. Lei,Sizheng Z. Lei,Huei-Sheng Vincent Chen,Huei-Sheng Vincent Chen,Nikolaus J. Sucher,Nikolaus J. Sucher,Joseph Loscalzo,Joseph Loscalzo,David J. Singel,Jonathan S. Stamler,Jonathan S. Stamler +15 more
TL;DR: It is reported that NO.-mediated neurotoxicity is engendered, at least in part, by reaction with superoxide anion (O.-2), apparently leading to formation of peroxynitrite (ONOO−), and not by NO.
Journal ArticleDOI
Nitric oxide directly activates calcium-dependent potassium channels in vascular smooth muscle
TL;DR: Evidence is presented that both exogenous nitric oxide and native EDRF can directly activate single Ca2+-dependent K+ channels (K+Ca) in cell-free membrane patches without requiring cGMP.
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Nitric oxide signaling in the central nervous system.
John Garthwaite,C L Boulton +1 more
TL;DR: The discovery that NO functions as a signaling molecule in the brain opened a new dimension in the concept of neural communication, one overlaying the classical picture of chemical neurotransmission, with a diffusive type of signal that disregards the spatial constraints on neu rotransmitter activity normally imposed by membranes, transporters, and in activating enzymes.
References
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Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
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A new generation of Ca2+ indicators with greatly improved fluorescence properties.
TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.
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Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
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Glycine potentiates the NMDA response in cultured mouse brain neurons
J. W. Johnson,P Ascher +1 more
TL;DR: G glycine may facilitate excitatory transmission in the brain through an allosteric activation of the NMDA receptor, and can be observed in outside-out patches as an increase in the frequency of opening of the channels activated by NMDA agonists.