Journal ArticleDOI
Effect of silver ions on transport and retention of phosphate by Escherichia coli.
W J Schreurs,H Rosenberg +1 more
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In the presence of N-ethylmaleimide and several uncouplers, Ag+ failed to cause phosphate efflux, but still inhibited exchange of intracellular and extracellular phosphate, indicating an interaction at more than one site.Abstract:
Silver ions inhibited phosphate uptake and exchange in Escherichia coli and caused efflux of accumulated phosphate as well as of mannitol, succinate, glutamine, and proline. The effects of Ag+ were reversed by thiols and, to a lesser extent, by bromide. In the presence of N-ethylmaleimide and several uncouplers, Ag+ failed to cause phosphate efflux, but still inhibited exchange of intracellular and extracellular phosphate, indicating an interaction at more than one site. It is unlikely that Ag+ caused metabolite efflux by acting solely as an uncoupler, as an inhibitor of the respiratory chain, or as a thiol reagent.read more
Citations
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Silver nanoparticles as a new generation of antimicrobials.
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Antiseptics and Disinfectants: Activity, Action, and Resistance
TL;DR: Known mechanisms of microbial resistance (both intrinsic and acquired) to biocides are reviewed, with emphasis on the clinical implications of these reports.
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Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells
TL;DR: A possible mechanism of toxicity is proposed which involves disruption of the mitochondrial respiratory chain by Ag-np leading to production of ROS and interruption of ATP synthesis, which in turn cause DNA damage.
Journal ArticleDOI
A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment
TL;DR: A review of the antibacterial effects of silver nanomaterials, including proposed antibacterial mechanisms and possible toxicity to higher organisms, is presented in this paper, where the authors suggest that further research is warranted given the already widespread and rapidly growing use of silver nanoparticles.
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Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli
TL;DR: The results of the present study suggest that silver ions may cause S. aureus and E. coli bacteria to reach an ABNC state and eventually die.
References
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Journal ArticleDOI
Chemiosmotic coupling in oxidative and photosynthetic phosphorylation
TL;DR: The end result of the coupling between the flows through the o/r and h/d pathways in oxidative phosphorylation in mitochondria is that, for the equivalent of each pair of electrons traversing the respiratory chain, up to 3 anhydro-bond equivalents may normally traverse the h/D pathway from adenosine diphosphate plus inorganic phosphate (ADP +Pi) to water.
Journal ArticleDOI
The effect of silver ions on the respiratory chain of Escherichia coli.
P. D. Bragg,D. J. Rainnie +1 more
TL;DR: Silver ions inhibited the oxidation of glucose, glycerol, fumarate, succinate, D- and L-lactate, and endogenous substrates by intact cell suspensions of Escherichia coli.
Journal ArticleDOI
Chemiosmotic coupling in oxidative and photosynthetic phosphorylation
TL;DR: This remarkable account of Peter Mitchell's ideas originally published in 1966 is a landmark and must-read publication for any scientist in the field of bioenergetics.
Journal ArticleDOI
Different Mechanisms of Energy Coupling for the Shock-sensitive and Shock-resistant Amino Acid Permeases of Escherichia coli
Edward A. Berger,Leon A. Heppel +1 more
TL;DR: It is concluded that the energy donors for the two classes of transport systems are fundamentally different and the obligatory requirement of phosphate bond energy for theshock-sensitive but not the shock-resistant permeases is suggested.
Journal ArticleDOI
Two systems for the uptake of phosphate in Escherichia coli.
TL;DR: A comparison of strains of Escherichia coli K-12 showed that one of the systems (PIT) was fully constitutive, required no binding protein, and operated in spheroplasts, while the other system, PST, was repressible by phosphate concentrations above 1 mM, required the phosphate-binding protein for full activity, and did not operate in sp Heroplasts.
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