eIF5A promotes translation of polyproline motifs.
Erik Gutierrez,Byung-Sik Shin,Christopher J. Woolstenhulme,Joo-Ran Kim,Preeti Saini,Allen R. Buskirk,Thomas E. Dever +6 more
TLDR
EIF5A, like its bacterial ortholog EFP, is proposed to stimulate the peptidyl transferase activity of the ribosome and facilitate the reactivity of poor substrates like Pro.About:
This article is published in Molecular Cell.The article was published on 2013-07-11 and is currently open access. It has received 392 citations till now. The article focuses on the topics: Translation factor & Hypusine.read more
Citations
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Functions of Polyamines in Mammals.
TL;DR: Studies with Gy mice and human patients with the very rare X-linked genetic condition Snyder-Robinson syndrome show clearly that the correct spermine:spermidine ratio is critical for normal growth and development.
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Dom34 Rescues Ribosomes in 3′ Untranslated Regions
Nicholas R. Guydosh,Rachel Green +1 more
TL;DR: It is suggested that ribosomes frequently enter downstream noncoding regions and that Dom34 carries out the important task of rescuing them.
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eIF5A Functions Globally in Translation Elongation and Termination
TL;DR: It is found that eIF5A strongly promotes the translation of the stalling sequences identified by profiling and increases the rate of peptidyl-tRNA hydrolysis more than 17-fold, rationalizing its high cellular abundance and essential nature.
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Upstream Open Reading Frames Differentially Regulate Gene-specific Translation in the Integrated Stress Response
Sara K. Young,Ronald C. Wek +1 more
TL;DR: This review presents key features of uORFs that serve to optimize translational control that is essential for regulation of cell fate in response to environmental stresses.
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Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use.
TL;DR: Together with dynamic codon redefinition, frameshifting is one of the forms of recoding that enriches gene expression and generates productively utilized products encoded trans-frame with respect to the genomic sequence.
References
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Genome-Wide Analysis in Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling
TL;DR: A ribosomesome-profiling strategy based on the deep sequencing of ribosome-protected mRNA fragments is presented and enables genome-wide investigation of translation with subcodon resolution and is used to monitor translation in budding yeast under both rich and starvation conditions.
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Structure of the 70S Ribosome Complexed with mRNA and tRNA
Maria Selmer,Christine M. Dunham,Frank V. Murphy,Albert Weixlbaumer,Sabine Petry,Ann C. Kelley,John R. Weir,Venki Ramakrishnan +7 more
TL;DR: The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (t RNA) and metal ions also stabilize the intersubunit interface.
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Translational control in cancer.
TL;DR: Clinical efforts are underway to target specific components of the translation apparatus or unique mRNA translation elements for cancer therapeutics to define a new understanding of the role of mRNA translation and protein synthesis in human cancer.
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What recent ribosome structures have revealed about the mechanism of translation
TL;DR: How the interaction between structural and functional studies over the last decade has led to a deeper understanding of the complex mechanisms underlying translation is discussed.
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Biochemical and genetic analysis of the yeast proteome with a movable ORF collection
Daniel M. Gelperin,Michael A. White,Martha L. Wilkinson,Yoshiko Kon,Li A. Kung,Kevin J. Wise,Nelson Lopez-Hoyo,Lixia Jiang,Stacy Piccirillo,Haiyuan Yu,Mark Gerstein,Mark E. Dumont,Eric M. Phizicky,Michael Snyder,Elizabeth J. Grayhack +14 more
TL;DR: A MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control, demonstrating that nearly all verified ORFs are expressed and suggesting the authenticity of 48 ORFs characterized as dubious.