Electrochemical Quantification of Neurotransmitters in Single Live Cell Vesicles Shows Exocytosis is Predominantly Partial
Ying Wang,Andrew G. Ewing +1 more
TLDR
Recent progress in amperometric measurements for monitoring exocytosis in single cells and electrochemical cytometry measurements of vesicular neurotransmitter content in individual vesicles are summarized.Abstract:
Exocytosis plays an essential role in the communication between cells in the nervous system. Understanding the regulation of neurotransmitter release during exocytosis and the amount of neurotransmitter content that is stored in vesicles is of importance, as it provides fundamental insights to understand how the brain works and how neurons elicit a certain behavior. In this minireview, we summarize recent progress in amperometric measurements for monitoring exocytosis in single cells and electrochemical cytometry measurements of vesicular neurotransmitter content in individual vesicles. Important steps have increased our understanding of the different mechanisms of exocytosis. Increasing evidence is firmly establishing that partial release is the primary mechanism of release in multiple cell types.read more
Citations
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Tracking the Electrocatalytic Activity of a Single Palladium Nanoparticle for the Hydrogen Evolution Reaction.
TL;DR: In this article, the authors take the hydrogen evolution reaction (HER) catalyzed by palladium nanoparticles as a model system to track the electrocatalytic activity of single Pd NPs by stochastic collision and ensemble electrochemistry, respectively.
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Nano-analysis Reveals High Fraction of Serotonin Release during Exocytosis from a Gut Epithelium Model Cell.
TL;DR: In this article, the authors explore the exocytotic nature of serotonin (5-HT) release in human carcinoid BON cells, an in-vitro human enterochromaffin cell model, to understand the mechanisms operating the release of gut-derived 5-HT in the intestinal mucosal epithelium.
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Anionic Species Regulate Chemical Storage in Nanometer Vesicles and Amperometrically Detected Exocytotic Dynamics
Xiulan He,Andrew G. Ewing +1 more
TL;DR: Amperometry has been applied to show that the vesicular content, dynamics of exocytosis and vesicles opening, depend on the anionic species treatment, providing understanding of the pharmacological and pathological processes induced by inorganic ions.
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Advances in microfluidic strategies for single-cell research
TL;DR: A review of microfluidic strategies for single-cell research relevant to tumorigenesis and evolution, immune responses, and drug discovery can be found in this article , where the authors discuss the design of microfluidic devices for singlecell analysis, the current methods of micro-fluidIC devices for individual cell isolation, and applications of microFluidics-based single-cells analysis in cell biology, disease diagnosis, and therapy.
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Single‐Vesicle Electrochemistry Following Repetitive Stimulation Reveals a Mechanism for Plasticity Changes with Iron Deficiency
Ying Wang,C. Gu,Andrew G. Ewing +2 more
TL;DR: Electrochemical methods show that during iron deficiency, repetitive stimulation causes significant decrease in exocytotic release without changing vesicular content, which suggests that iron deficiency has a negative effect on plasticity by decreasing the fraction of vesicle release in response to repetitive stimulation.
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