Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM
Xueming Li,Paul Mooney,Shawn Q. Zheng,Shawn Q. Zheng,Christopher R. Booth,Michael B. Braunfeld,Michael B. Braunfeld,Sander Gubbens,David A. Agard,David A. Agard,Yifan Cheng +10 more
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TLDR
This approach determined a 3.3-Å-resolution structure of an ∼700-kDa protein with D7 symmetry, the Thermoplasma acidophilum 20S proteasome, showing clear side-chain density and greatly enhances image quality and data acquisition efficiency.Abstract:
In recent work with large high-symmetry viruses, single-particle electron cryomicroscopy (cryo-EM) has achieved the determination of near-atomic-resolution structures by allowing direct fitting of atomic models into experimental density maps. However, achieving this goal with smaller particles of lower symmetry remains challenging. Using a newly developed single electron-counting detector, we confirmed that electron beam-induced motion substantially degrades resolution, and we showed that the combination of rapid readout and nearly noiseless electron counting allow image blurring to be corrected to subpixel accuracy, restoring intrinsic image information to high resolution (Thon rings visible to ∼3 A). Using this approach, we determined a 3.3-A-resolution structure of an ∼700-kDa protein with D7 symmetry, the Thermoplasma acidophilum 20S proteasome, showing clear side-chain density. Our method greatly enhances image quality and data acquisition efficiency-key bottlenecks in applying near-atomic-resolution cryo-EM to a broad range of protein samples.read more
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New Insights on the Structure of Electrochemically Deposited Lithium Metal and Its Solid Electrolyte Interphases via Cryogenic TEM
Xuefeng Wang,Minghao Zhang,Judith Alvarado,Shen Wang,Mahsa Sina,Bingyu Lu,James C. Bouwer,Wu Xu,Jie Xiao,Ji-Guang Zhang,Jun Liu,Ying Shirley Meng +11 more
TL;DR: Inspired by biological imaging techniques, this work demonstrates the power of cryogenic (cryo)-electron microscopy to reveal the detailed structure of EDLi and the SEI composition at the nanoscale while minimizing beam damage during imaging.
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TL;DR: Cryo–electron microscopy structures of a supercomplex consisting of a dimeric core and two strongly bound and two moderately bound LHCIIs (C2S2M2), is reported, which is the dominant form in plants acclimated to limited light.
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Structure of a modular polyketide synthase
Somnath Dutta,Jonathan R. Whicher,Douglas A. Hansen,Wendi A. Hale,Joseph A. Chemler,Grady R. Congdon,Alison R. H. Narayan,Kristina Håkansson,David H. Sherman,Janet L. Smith,Georgios Skiniotis +10 more
TL;DR: This study reveals for the first time the structural basis for both intramodule and intermodule substrate transfer in polyketide synthases, and establishes a new model for molecular dissection of these multifunctional enzyme systems.
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